Current Genetics

, Volume 2, Issue 3, pp 193–200 | Cite as

Control of recombination within and between DNA plasmids of Saccharomyces cerevisiae

  • Melanie J. Dobson
  • A. Bruce Futcher
  • Brian S. Cox
Article

Summary

[2 μm+ and [2μm°] yeast were transformed to stable leucine prototrophy with the hybrid yeast — E. coli plasmid, pJDB219. This plasmid contains the entire sequence of the endogenous 2 μm yeast DNA plasmid in addition to the yeast nuclear LEU2+ gene and the Co1E1 derivative, pMB9. In the [2 μm+] transformants, a new wholly yeast LEU2+ plasmid, pYX, was generated, probably by a recombination event between pJDB219 and 2 μm DNA. The plamid, pYX, in the absence of 2 μm DNA, was found to exist in equimolar amounts of two forms, A and B, which probably arise by intramolecular recombination across the inverted repeat sequences of the 2 μm DNA portion of the plasmid. pJDB219 was found to require the presence of 2 μm DNA to undergo this intramolecular recombination. The results suggest that 2, μm DNA and pYX code for a gene product required in this recombination event which pJDB219 cannot produce.

Key words

Recombination Plasmids Transformation Yeast 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Melanie J. Dobson
    • 1
  • A. Bruce Futcher
    • 1
  • Brian S. Cox
    • 1
  1. 1.Botany SchoolOxfordEngland

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