Summary
Newly synthesised DNA molecules the same size as replicons (7 million-60 million daltons) accumulate in yeast cells treated with hydroxyurea. During prolonged incubation in low concentrations of the drug, there is a large accumulation of these molecules without any corresponding increase in their molecular weight. On release from the inhibtion the molecules are converted to large molecular weight DNA. These observations are consistent with an inhibition by hydroxyurea of the joining of completed replicons. In addition, newly synthesised DNA molecules the size of yeast Okazaki fragments also accumulate in cells treated with hydroxyurea.
Similar content being viewed by others
References
Anderson MLM (1978) Nucleic Acids Res 5:4343–4354
Burg K, Collins ARS, Johnson RL (1977) J Cell Sci 28:29–48
Chang LMS (1977) J Biol Chem 252:1873–1880
Collins ARS (1977) Biochim Biophys Acta 478:461–473
Collins ARS, Schor SL, Johnson RL (1977) Mutat Res 42:413–432
Culotti J, Hartwell LH (1971) Exp Cell Res 67:389–901
Elford HL (1968) Biochem Biophys Res Commun 33:129–135
Hartwell LH (1976) J Mol Biol 104:803–817
Johnston LH, Game JC (1978) Mol Gen Genet 61:205–214
Johnston LH, Nasmyth KA (1978) Nature 274:891–893
Johnston LH, Williamson DH (1978) Mol Gen Genet 164:217–225
Levin D, Hutchinson F (1973) J Mol Biol 75:495–502
Petes TD, Williamson DH (1975) Exp Cell Res 95:103–110
Sinha NK, Snustad DP (1972) J Bacteriol 112:1321–1334
Slater ML (1973) J Bacteriol 113:263–270
Timson J (1975) Mutat Res 32:115–132
Tye B, Nyman P, Lehman IR, Hochhauser S, Weiss B (1977) Proc Natl Acad Sci USA 74:154–157
Wintersburger E (1974) Eur J Biochem 50:41–47
Yarranton GT, Moore PD, Spanos A (1976) Mol Gen Genet 145:215–218
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Johnston, L.H. The effect of hydroxyurea on the mechanism of DNA synthesis in the yeast Saccharomyces cerevisiae . Curr Genet 2, 175–180 (1980). https://doi.org/10.1007/BF00435682
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00435682