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Labelling monoclonal antibodies with Yttrium-90

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Abstract

Using the cyclic DTPA derivatisation procedure developed by Hnatowich, conditions have been optimised for labelling three tumour-associated monoclonal antibodies with 90Y. High labelling efficiencies (>95%) at modest specific activities (1 mCi/mg) could be routinely obtained. Radiochemical stability of the radiolabelled preparations in vitro was good, but radiolysis resulted in early losses of antibody immunoreactivity.

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Abbreviations

PBS:

Phosphate buffered saline

BSA:

Bovine Serum Albumin

HSA:

Human Serum Albumin

ITLC:

Instant thin layer chromatography

HPLC:

High pressure liquid chromatography

CAE:

Cellulose acetate electrophoresis

ELISA:

Enzyme linked immunosorbent assay

RIA:

Radioimmune assay

DTPA:

Diethylenetriaminepentaacetic acid

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Authors and Affiliations

  1. Department of Nuclear Medicine, St. Bartholomews Hospital, EC1A 7BE, London, UK

    Stephen J. Mather

  2. Unipath Ltd, Bedford, UK

    David M. Tolley & G. Wesley White

Authors
  1. Stephen J. Mather
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  2. David M. Tolley
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  3. G. Wesley White
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Mather, S.J., Tolley, D.M. & White, G.W. Labelling monoclonal antibodies with Yttrium-90. Eur J Nucl Med 15, 307–312 (1989). https://doi.org/10.1007/BF00435471

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  • DOI: https://doi.org/10.1007/BF00435471

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