Current Genetics

, Volume 15, Issue 2, pp 155–160 | Cite as

Partial characterization of the gene coding for subunit IV of soybean mitochondrial NADH dehydrogenase

  • Henri Wintz
  • Hsu-Ching Chen
  • Datta T. N. Pillay
Original Articles

Summary

By using the spinach chloroplast atpE gene (ε-subunit coding gene) as a probe we have isolated, from a soybean mitochondrial DNA library, a sequence containing a 405 base-pairs (bp) open-reading frame (ORF). This ORF, which is unique in the soybean mitochondrial genome, is probably part of an exon of the gene coding for subunit IV of the NADH dehydrogenase complex. The predicted protein shows 42% sequence similarity with the C-terminal region the Aspergillus nidulans NAD4 protein. The gene is split by a class II intron which is larger than 1,950 bp. Transcription analysis revealed a single 2,200 nucleotide long transcript which does not contain the intron sequence.

Key word

Mitochondrion Genome Plant NADH dehydrogenase Intron 

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References

  1. Anderson S, Bankier AT, Barrell BG, De Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreir PH, Smith AJH, Staden, Young IG (1981) Nature 290:457–465Google Scholar
  2. Bibb MJ, Von Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Cell 26:167–180Google Scholar
  3. Attardi G (1985) Nature 314:592–597Google Scholar
  4. Dawson AJ, Jones VP, Leaver CJ (1984) EMBO J 3:2107–2113Google Scholar
  5. Dewey RE, Levings CS III, Timothy DH (1985a) Plant Physiol 79:914–919Google Scholar
  6. Dewey RE, Schuster AM, Levings CS III, Timothy DH (1985b) Proc Natl Acad Sci USA 82:1015–1019Google Scholar
  7. Dewey RE, Levings CS III, Timothy DH (1986) Cell 44:439–449Google Scholar
  8. Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) Mol Gen Genet 215:118–127Google Scholar
  9. Fox TD, Leaver CJ (1981) Cell 26:315–323Google Scholar
  10. Hiesel R, Schobel W, Schuster W, Brennicke A (1987) EMBO J 6:29–34Google Scholar
  11. Isaac PG, Jones VP, Leaver CJ (1985) EMBO J 4:1617–1623Google Scholar
  12. Ise W, Haiker H, Weiss H (1985) EMBO J 4:2075–2080Google Scholar
  13. Leaver CJ, Forde BG (1980) In: Leaver CJ (ed) Genome organisation and expression in plants. Plenum Press, New York, pp 407–425Google Scholar
  14. Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
  15. McMaster GK, Carmichael GG (1977) Proc Natl Acad Sci USA 74:4835–4838Google Scholar
  16. Michel F, Dujon B (1983) EMBO J 2:33–38Google Scholar
  17. Nelson MA, Macino G (1987a) Mol Gen Genet 206:307–317Google Scholar
  18. Nelson MA, Macino G (1987b) Mol Gen Genet 206:318–325Google Scholar
  19. Newton KJ (1988) Annu Rev Plant Physiol Plant Mol Biol 39:503–532Google Scholar
  20. Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  21. Schuster W, Brennicke A (1987) EMBO J 6:2857–2863Google Scholar
  22. Stern DB, Bang AG, Thompson WF (1986) Curr Genet 10:857–869Google Scholar
  23. Stern DB, Newton KJ (1986) Methods Enzymol 118:488–496Google Scholar
  24. Sugiura M, Shinozaki K, Tanaka M, Hayashida N, Wakasugi T, Matsubayashi T, Ohto C, Torazawa K, Meng BY, Hidaka T, Zaita N (1987) In: D Von Wettstein, Chua NH (eds) Plant molecular biology. Plenum Press, pp 65–76Google Scholar
  25. Wintz H, Skunca M, Pillay DTN (1987) Gene 59:47–53Google Scholar
  26. Wintz H, Chen HC, Pillay DTN (1988) Curr Genet 13:255–260Google Scholar
  27. Wissinger B, Hiesel R, Schuster W, Brennicke A (1988) Mol Gen Genet 212:56–65Google Scholar
  28. Young EG, Hanson MR (1987) Cell 50:41–49Google Scholar
  29. Zurawsky G, Bottomley W, Whitfeld PR (1982) Proc Natl Acad Sci USA 79:6260–6264Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Henri Wintz
    • 1
  • Hsu-Ching Chen
    • 1
  • Datta T. N. Pillay
    • 1
  1. 1.Department of Biological SciencesUniversity of WindsorWindsorCanada

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