Summary
Purine rich small “RNA-primer” molecules (about 10–12 nucleotides), secreted into the growth medium of 3-h germinated conidia of N. crassa, strongly stimulated a concentration-dependent in vitro DNA synthesis of N. crassa slime mutant as well as DNAs from the human cancer cells but did not affect that from normal cells. These “RNA-primer” molecules stimulated also in vivo cell growth of N. crassa slime mutant, but not of the N. crassa wild type. Our studies suggest that DNAs from the slime mutant of N. crassa as well as DNAs from human cancerous cells provide increased sites for enhanced in vitro and in vivo replication of DNAs. “RNA-primer” molecules can be hydrolyzed by T1 RNase but not by pancreatic RNase.
Similar content being viewed by others
References
Andrews P (1965) Biochim J 96:595
Beljanski M (1983) In: Wolsky A (ed) Experimental biology and medicine, vol 8. Karger, New York
Beljanski M, Beljanski MS (1974) Biochem Genet 12:163
Beljanski M, Le Goff L (1979) C R Acad Sci Paris (Ser D) 288:147
Beljanski M, Le Goff L (1986) In: Dutta SK (ed) DNA systematics: Evolution, vol I. CRC Press, Florida, p 81
Beljanksi M, Beljanski MS, Manigault P, Bourgarel P (1972) Proc Natl Acad Sci USA 69:191
Beljanski M, Bourgarel P, Beljanski MS (1981) Expl Cell Biol 49:220
Beljanski M, Plawecki M, Bourgarel P, Beljanski MS (1983) Cancer Treat Rep 67:611
Bjork R, Svenson I (1967) Biochim Biophys Acta 138:430
Champoux JJ, Gilboa E, Baltimore D (1984) J Virol 49:686
Chaudhuri RK, Dutta SK, Ojha M (1973) Oncology 28:553
Dutta SK, Sheikh I, Choppala J, Aulakh GS, Nelson WH (1976) Mol Gen Genet 189:207
Dutta SK, Williams NP, Mukhopadhyay DK (1983) Mol Gen Gent 189:207
Emerson S (1963) Genetica 34:162
Fox RM, Mendelson J, Barbosa E, Goulian M (1973) Nature (London) New Biol 235:234
Hori M (1967) In: Grossman J, Moldave K (eds) Methods in enzymology, vol XII, part A. Nucleic acids. Academic, New York, p 381
Lee-Huang S, Sierra JM, Naranjo R, Filipowitz W, Ochoa S (1977) Archs Biochem Biophys 180:276
Mukhopadhyay DK, Dutta SK (1979) Indian J Exp Biol 17:620
Plawecki M, Beljanski M (1974) C R Acad Sci Paris (Ser D) 278:1413
Roven L, Komberg A (1978) J Biol Chem 253:770
Schekman R, Wickner W, Westergaard O, Brutlag D, Geider K, Bertsh L, Konrberg A (1972) Proc Natl Acad Sci USA 69:2691
Smith JK, Cywinski A, Taylor JM (1984) J Virol 52:314
Tyler BM, Giles NH (1985) Nucleic Acids Res 13:4311
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dutta, S.K., Beljanski, B. Particular RNA primer from growth medium differentially stimulates in vitro DNA synthesis and in vivo cell growth of Neurospora crassa and its slime mutant. Curr Genet 12, 283–289 (1987). https://doi.org/10.1007/BF00435291
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00435291