Advertisement

Current Genetics

, Volume 12, Issue 4, pp 251–256 | Cite as

Chloroplast gene transmission in Chlamydomonas reinhardtii. A model for its control by the mating-type locus

  • René F. Matagne
Original Articles

Summary

In sexual crosses of Chlamydomonas reinhardtii, genes residing in the chloroplast (cp) are most often transmitted from the mating-type plus (mt+) parent only. Galloway and Goodenough (1985) proposed a model in which the mt locus (linkage group VI) is a complex region containing several genes involved in the control of both gametic differentiation and cp inheritance. The mt+ locus contains: the sfu locus necessary for sexual fusion between gametes; the upp locus (uniparental plus) which controls cp gene inheritance and also perhaps zygote maturation; and the sad locus which functions in sexual adhesion. The mt locus also contains a sad locus as well as a regulatory element (mid) necessary for the minus dominance in mt+/mt diploid gametes. This model has been extended to include new genetic functions linked to the mt+ or mt locus. In this new system, there is a group of genes (maps for mating-type plus structure), present in both plus and minus strains, controlling some mt+ phenotypical traits as well as the synthesis of an activator of the cp DNA nuclease; two genes contained in the mt locus — one (cge for cp genomic elimination) coding for a nuclease (in an inactive form) located in the chloroplast and another (mid) coding for a repressor of maps; one gene (upp) contained in the mt+ locus, coding for a substance preventing the synthesis or the activity of the nuclease and perhaps also acting on the maps product. The model can be applied to explain the complex dominance/ recessivity relationships observed between the mt+ and mt “alleles” in heterozygous diploid gametes, the basis for mitotic vs meiotic zygote formation and the differences in transmission observed for these two physiologically distinct zygote classes. Application of the model for prediction of mutant phenotypes and the design of future experiments is also described.

Key words

Chlamydomonas reinhardtii Chloroplast heredity Mating-type locus 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adams GMW (1982) Curr Genet 5:1–3Google Scholar
  2. Bergman K, Goodenough UK, Goodenough DA, Jawitz J, Martin H (1975) J Cell Biol 67:606–622Google Scholar
  3. Burton WG, Grabowy CT, Sager R (1979) Proc Natl Acad Sci USA 76:1390–1394Google Scholar
  4. Cavalier-Smith T (1970) Nature 228:333–335Google Scholar
  5. Eves EM, Chiang KS (1984) Genetics 107:563–576Google Scholar
  6. Feng TY, Chiang KS (1984) Proc Natl Acad Sci USA 81:3438–3442Google Scholar
  7. Forest CL, Togasaki RK (1975) Proc Natl Acad Sci USA 72: 3652–3655Google Scholar
  8. Galloway RE, Goodenough UW (1985) Genetics 111:447–461Google Scholar
  9. Galloway RE, Holden LR (1984) Curr Genet 8:399–405Google Scholar
  10. Gillham NW (1963) Nature 200:294Google Scholar
  11. Gillham NW, Boynton JE, Johnson AM, Burkhart BD (1987) Genetics 115:677–684Google Scholar
  12. Goodenough NW, Detmers PA, Hwang C (1982) J Cell Biol 92: 378–386Google Scholar
  13. Goodenough UW, Hwang CJ, Warren AJ (1978) Genetics 89: 235–243Google Scholar
  14. Grant DM, Gillham NW, Boynton JE (1980) Proc Natl Acad Sci USA 77:6067–6071Google Scholar
  15. Hwang CJ, Monk BC, Goodenough UW (1981) Genetics 99:41–47Google Scholar
  16. Kuroiwa T, Kawano S, Nishibayashi S, Sato C (1982) Nature 298:481–483Google Scholar
  17. Matagne RF (1981) Curr Genet 3:31–36Google Scholar
  18. Matagne RF, Beckers M-C (1983) Curr Genet 7:335–338Google Scholar
  19. Matagne RF, Hermesse M-P (1980) Curr Genet 1:127–131Google Scholar
  20. Matagne RF, Hermesse M-P (1981) Genetics 99:371–381Google Scholar
  21. Matagne RF, Mathieu D (1983) Proc Natl Acad Sci USA 80: 4780–4783Google Scholar
  22. Matagne RF, Schaus M (1985) Curr Genet 10:81–85Google Scholar
  23. Matagne RF, Yu V (1987) Curr Genet 11:605–610Google Scholar
  24. Matsuda Y, Sakamoto K, Tsubo Y (1983) Curr Genet 7:339–345Google Scholar
  25. Royer HD, Sager R (1979) Proc Natl Acad Sci USA 76:5794–5798Google Scholar
  26. Sager R (1954) Proc Natl Acad Sci USA 40:356–363Google Scholar
  27. Sager R, Grabowy C, Sano H (1981) Cell 24:41–47Google Scholar
  28. Sager R, Ramanis Z (1973) Theor Appl Genet 43:101–108Google Scholar
  29. Sager R, Sand H, Grabowy CT (1984) Curr Topics Microbiol Immunol 108:157–172Google Scholar
  30. Tsubo Y, Matsuda Y (1984) Curr Genet 8:223–229Google Scholar
  31. VanWinkle-Swift KP (1978) Nature 275:749–750Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • René F. Matagne
    • 1
  1. 1.Genetics of Microorganisms, Department of Botany, B-22University of LiégeLiégeBelgium

Personalised recommendations