Current Genetics

, Volume 6, Issue 2, pp 171–172 | Cite as

Cleavage of the double stranded RNA genome of killer yeast

  • Friedhelm Meinhardt
Short Communication


An enzyme splitting ribonucleic acid was isolated from the bakers yeast Saccharomyces cerevisiae and partially purified by differential ammonium sulfate precipitation followed by gel filtration.

The enzyme cleaves killer double stranded RNA from yeast (MW 1.1 × 106 D) into two distinct pieces (MW 0.62 × 106 and 0.43 × 106 D) as revealed by gel electrophoresis.

This enzyme thus resembles the action of restriction endonucleases used for cleaving DNA and might therefore be used in transformation experiments with double stranded RNA.

Key words

Killer plasmid Restriction RNAse 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Friedhelm Meinhardt
    • 1
  1. 1.Lehrstuhl für Allgemeine BotanikRuhr-Universität BochumBochumGermany

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