Cleavage of the double stranded RNA genome of killer yeast
- 25 Downloads
An enzyme splitting ribonucleic acid was isolated from the bakers yeast Saccharomyces cerevisiae and partially purified by differential ammonium sulfate precipitation followed by gel filtration.
The enzyme cleaves killer double stranded RNA from yeast (MW 1.1 × 106 D) into two distinct pieces (MW 0.62 × 106 and 0.43 × 106 D) as revealed by gel electrophoresis.
This enzyme thus resembles the action of restriction endonucleases used for cleaving DNA and might therefore be used in transformation experiments with double stranded RNA.
Key wordsKiller plasmid Restriction RNAse
- Bozarth RF (1979) In: Lemke PA (ed) Viruses and Plasmids in Fungi. Marcel Dekker, New York, pp 44–87Google Scholar
- Castanho B, Buttler EE, Shepherd RJ (1978) Phytopathology 68:1515–1519Google Scholar
- Fink GR, Styles CA (1972) Proc Natl Acad Sci USA 69:2846–2849Google Scholar
- Fried HM, Fink GR (1978) Proc Natl Acad Sci USA 75:4224–4228Google Scholar
- Franklin RM (1966) Proc Natl Acad Sci USA 55:1504–1511Google Scholar
- Koltin Y, Day PR (1976) Proc Natl Acad Sci USA 73:594–598 598Google Scholar
- Lemke PA (1977) In: Schlesinger D (ed) Microbiology 1977. Am Soc Microbiol, Wash. DC, pp 44–87Google Scholar
- Welsh JD, Leibowitz MJ (1980) Nucleic Acids Res 8:2365–2375Google Scholar
- Welsh JD, Leibowitz MJ (1982) Proc Natl Acad Sci USA 79:786–789Google Scholar
- Wickner RB (1979) Plasmid 2:303–322Google Scholar