Current Genetics

, Volume 6, Issue 2, pp 153–158 | Cite as

Extrachromosomal genetics of Cephalosporium acremonium

II. Development of a mitochondrial DNA hybrid vector replicating in Saccharomyces cerevisiae
  • Paul Tudzynski
  • Karl Esser
Article

Summary

In order to facilitate strain improvement by concerted breeding in the cephalosporin producing imperfect filamentous fungus Cephalo sporium acremonium, it is attempted to develop a eukaryotic vector for molecular cloning based on mitochondrial (mt) DNA.

Fragments of mtDNA from C. acremonium were inserted into a yeast/bacterial hybrid plasmid (pDAM1) lacking a eukaryotic replicon. Six hybrid plasmids were obtained each containing a different mt fragment which together comprise about 60% of the total mtDNA. One of these recombinant plasmid vectors (pCP2) showed a high replication efficiency which is comparable to that of vectors containing yeast 2 μm DNA. This plasmid therefore fulfills the requirements for practical application.

Key words

Cephalosporium acremonium Eukaryotic Hybrid vector mtDNA 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Paul Tudzynski
    • 1
  • Karl Esser
    • 1
  1. 1.Lehrstuhl für Allgemeine BotanikRuhr-UniversitätBochum 1Germany

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