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The recovery, penetrance, and pleiotropy of X-linked, cold sensitive mutants in Drosophila

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Summary

The viability at 16°, 22°, and 30°C, loci, visible phenotypes if any, possible effective lethal phase, and female fertility of seven X-linked, recessive cold sensitive mutations are reported. Five of the seven are female sterile at the restrictive temperature of 16°C; two of these five are also female sterile at the permissive temperature of 25°C. For two of the five mutations which are female sterile, escapers at the restrictive temperature exhibit visible phenotypes characteristic of mutants which affect protein synthesis. The possibility that some of the mutants affect ribosomes is considered. One of the mutants, l(1)TW-6 cs, is probably a cold sensitive meiotic mutant as well as a cold sensitive zygotic lethal. One of the mutants is a non-conditional visible allele of lozenge.

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References

  • Baillie, D., Suzuki, D. T., Tarasoff, M.: Temperature-sensitive mutations in Drosophila melanogaster. II. Frequency among second chromosome recessive lethals induced by ethyl methanesulfonate. Canad. J. Genet. Cytol. 10, 412–420 (1968).

    Google Scholar 

  • Dudick, M.: A temperature-sensitive allele of a suppressor gene in Drosophila. Master's Thesis, University of Virginia, Charlottesville, Va. (1973).

    Google Scholar 

  • Dudick, M., Wright, T. R. F.: A temperature-sensitive lethal allele of suppressor of forked in Drosophila. (In Press.)

  • Guthrie, C., Nashimoto, H., Nomura, M.: Structure and function of E. coli ribosomes. VIII. Cold-sensitive mutants defective in ribosome assembly. Proc. nat. Acad. Sci. (Wash.) 63, 384–391 (1969).

    Google Scholar 

  • Hillman, R.: A53g: Abnormal53g (Abnormal Abdomen53g). Drosophila Inform. Serv. 27, 56 (1953).

    Google Scholar 

  • Lambertsson, A. G., Rasmuson, S. B.: Studies on the ribosomes and ribosomal proteins of Drosophila melanogaster. Hereditas (Lund) 69, 299 (1971).

    Google Scholar 

  • Lindsley, D. L., Grell, E. H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Washington Publ. 627 (1968).

  • Mohan, J., Ritossa, F. M.: Regulation of ribosomal RNA synthesis and its bearing on the bobbed phenotype in Drosophila melanogaster. Develop. Bio. 22, 495–512 (1970).

    Google Scholar 

  • O'Donovan, G. A., Ingraham, J. L.: Cold-sensitive mutants of Escherichia coli resulting from increased feedback inhibition. Proc. nat. Acad. Sci. (Wash.) 54, 451–457 (1965).

    Google Scholar 

  • Ritossa, F. M., Atwood, K. C., Lindsley, D. L., Spiegelman, S.: On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA. Nat. Cancer Inst. Monogr. 23, 449–472 (1965).

    Google Scholar 

  • Rose, R., Hillman, R.: In vitro studies of protein synthesis in Drosophila. Biochem. biophys. Res. Commun. 35, 197–204 (1969).

    Google Scholar 

  • Rosenbluth, R., Ezell, D., Suzuki, D. T.: Temperature-sensitive mutations in Drosophila melanogaster. IX. Dominant cold-sensitive lethals on the autosomes. Genetics 70, 75–86 (1972).

    Google Scholar 

  • Rosenbluth, R., Mahoh, H., Ezell, D., Willis, W., Somero, M.: Cold sensitive lethal mutations in Drosophila melanogaster. Canad. J. Genet. Cytol. 12, 394 (1970).

    Google Scholar 

  • Schalet, A.: The suppressor of forked locus: seven characteristics in search of a molecular author. Third European Drosophila Res. Conf. Abstracts. In Press (1973).

  • Schalet, A., Singer, K.: A revised map of genes in the proximal region of the X chromosome of Drosophila melanogaster. Drosophila Inform. Serv. 46, 131–132 (1971).

    Google Scholar 

  • Steffensen, D. M., Wimber, D. E.: Localization of tRNA genes in the salivary chromosomes of Drosophila by RNA:DNA hybridization. Genetics 69, 163–178 (1971).

    Google Scholar 

  • Suzuki, D. T.: Temperature-sensitive mutations in Drosophila melanogaster. Science 170, 695–706 (1970).

    Google Scholar 

  • Suzuki, D. T., Procunier, D.: Temperature-sensitive mutations in Drosophila melanogaster. III. Dominant lethals and semilethals on Chromosome 2. Proc. nat. Acad. Sci. (Wash.) 62, 369–376 (1969).

    Google Scholar 

  • Tai, P.-C., Kessler, D. P., Ingraham, J.: Cold sensitive mutations in Salmonella typhimurium which affect ribosome synthesis. J. Bact. 97, 1298–1304 (1969).

    Google Scholar 

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Communicated by E. Hadorn

Supported by NSF Grants GB 7707 and GB 20910.

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Wright, T.R.F. The recovery, penetrance, and pleiotropy of X-linked, cold sensitive mutants in Drosophila . Molec. Gen. Genet. 122, 101–118 (1973). https://doi.org/10.1007/BF00435184

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