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DNA-binding proteins of the transcriptionally active chromosome from mustard (Sinapis alba L.) chloroplasts

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Summary

By using a blotting and incubation procedure with radioactive DNA probes, several protein species capable of high-affinity DNA binding have been detected among the proteins associated with mustard (Sinapis alba) chloroplast transcription complexes. Species with different affinity for single- and double-stranded DNA have been distinguished by using probes labelled by two different procedures in combination with unlabelled competitor DNA. Proteins of Mr 145,000, 125,000, 115,000, and 33,000 bind strongly to single-stranded DNA, while those of Mr 81,000, 57,000, 19,000, and 17,000 appear to bind both single- and double-stranded DNA.

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Abbreviations

bp :

base pair(s)

psbA :

gene encoding the herbicide-binding Mr 32,000 QB-protein of photosystem II

SDS :

sodium dodecyl sulfate

TAC :

transcriptionally active chromosome

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Author notes

  1. Thomas Reiss

    Present address: MSU-DOE Plant Research Laboratory, Michigan State University, 48824-1312, East Lansing, MI, USA

Authors and Affiliations

  1. Plant Cell Physiology, Ruhr-University Bochum, P.O. Box 102148, D-4630, Bochum 1, Germany

    Sven Bülow, Thomas Reiss & Gerhard Link

Authors
  1. Sven Bülow
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  2. Thomas Reiss
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Bülow, S., Reiss, T. & Link, G. DNA-binding proteins of the transcriptionally active chromosome from mustard (Sinapis alba L.) chloroplasts. Curr Genet 12, 157–159 (1987). https://doi.org/10.1007/BF00434671

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  • DOI: https://doi.org/10.1007/BF00434671

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