Summary
The mitochondrial DNA from seven species of the aquatic phycomycete Allomyces has been isolated and characterized by restriction enzyme analysis. Comparison of the mitochondrial DNA restriction enzyme fragmentation patterns showed pronounced differences not only among species but also among four isolates of A. arbuscula. The mitochondrial DNAs range in size from 39 kbp in A. neo-moniliformis to 56 kbp in A. macrogynus.
A physical map of the mitochondrial DNA of Allomyces arbuscula strain Costa Rica 21 has been constructed. The genome is circular and has a size of 49.2 kbp. The genes coding for the small and large ribosomal RNAs, cytochrome oxidase subunits 1, 2, and 3, apocytochrome b, and ATPase subunits 6 and 9 were localized in the mitochondria) DNA by heterologous hybridization with specific mitochondria) gene probes from Saccaromyces cerevisiae and Neurospora crassa. Comparison of the gene map of the closely related species Blastocladiella emersonii with that of A. arbuscula indicates a similar gene order in the two organisms.
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References
Borkhardt B, Delius H (1983) Curr Genet 7:327–333
Borkhardt B, Olson LW (1986) Curr Genet 11:139–143
Boyd DA, Hobman JC, Gruenke SA, Klassen GR (1984) Can J Biochem Cell Biol 62:571–576
Burger G, Scriven C, Machleidt W, Werner S (1982) EMBO J 1:1385–1391
Collins RA, Lambowitz AM (1983) Plasmid 9:53–70
Dujon B (1983) Mitochondrial genes mutants and maps: a review. In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983: nucleo-mitochondrial interactions. de Gruyter, Berlin New York, pp 1–24
Earl AJ, Turner G, Croft JH, Dales BG, Lazarus CM, Lunsdorf H, Kuntzel H (1981) Curr Genet 3:221–228
Garber RC, Yoder OC (1984) Curr Genet 8:621–628
Hintz WE, Mohan M, Anderson JB, Horgen PA (1985) Curr Genet 9:127–132
Hudspeth MES, Shumard DS, Bradford CJR, Grossman LI (1983) Proc Natl Acad Sci USA 80:142–146
Klimczak LJ, Prell HH (1984) Curr Genet 8:323–326
Kozlowski M, Stepien PP (1982) J Gen Microbiol 128:471–476
Lang BF, Wolf K (1984) Mol Gen Genet 196:465–472
Maniatis T, Fritsch EF, Sambrook J (1982) Cold Spring Harbor Laboratory, Cold Spring Harbor
Sanders JPM, Heyting C, Verbeet MPH, Meulink FCPW, Borst P (1977) Mol Gen Genet 157:239–261
Sederoff RR (1984) Structural variation in mitochondrial. In. Scandalios JG (ed) Advances in genetics, vol 22. Molecular genetics of plants. Academic, New York London, pp 1–108
Specht CA, Novotny CP, Ullrich RC (1983) Exp Mycol 7:336–343
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Borkhardt, B., Pedersen, M.B. & Olson, L.W. Mitochondrial DNA in the aquatic fungus Allomyces . Curr Genet 12, 149–156 (1987). https://doi.org/10.1007/BF00434670
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DOI: https://doi.org/10.1007/BF00434670