Current Genetics

, Volume 12, Issue 2, pp 99–104 | Cite as

Terminal segment of Kluyveromyces lactis linear DNA plasmid pGKL2 supports autonomou sreplication of hybrid plasmids in Saccharomyces cerevisiae

  • Hiro-aki Fujimura
  • Fumio Hishinuma
  • Norio Gunge
Original Articles


By use of linear DNA plasmid pGKL2 from the yeast Kluyveromyces lactis we have constructed hybrid plasmids carrying a LEU2 gene of Saccharomyces cerevisiae as a selectable marker. The replication properties of hybrid plasmids in yeasts were investigated. We demonstrated that the insertion of a LEU2 gene into pGKL2 resulted in circularization of the hybrid plasmids and pGKL2 segment supported autonomous replication of the plasmids. Moreover, the hybrid plasmids propagated autonomously, independently of the presence of the natural pGKL2 plasmid.

Key words

ARS Linear DNA killer plasmid Replication Saccharomyces cerevisiae 


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Hiro-aki Fujimura
    • 1
  • Fumio Hishinuma
    • 1
  • Norio Gunge
    • 1
  1. 1.Mitsubishi Kasei Institute of Life SciencesMinamiooya, Machidashi, TokyoJapan

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