Summary
By use of linear DNA plasmid pGKL2 from the yeast Kluyveromyces lactis we have constructed hybrid plasmids carrying a LEU2 gene of Saccharomyces cerevisiae as a selectable marker. The replication properties of hybrid plasmids in yeasts were investigated. We demonstrated that the insertion of a LEU2 gene into pGKL2 resulted in circularization of the hybrid plasmids and pGKL2 segment supported autonomous replication of the plasmids. Moreover, the hybrid plasmids propagated autonomously, independently of the presence of the natural pGKL2 plasmid.
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References
Broach JR, Li Y-Y, Feldman J, Yayaram M, Abraham J, Nasmyth KA, Hicks JB (1982) Cold Spring Harbor Symp Quant Biol 47:1165–1173
Clarke L, Carbon J (1980) Nature 287:504–509
Davis RW, Botstein D, Both JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Froman BE, Tait RC, Rodriguez RL (1984) Gene 31:257–261
Gunge N, Sakaguchi K (1981) J Bacteriol 147:155–160
Gunge N, Yamane C (1984) J Bacteriol 159:533–539
Gunge N, Tamaru A, Ozawa F, Sakaguchi K (1981) J Bacteriol 145:382–390
Gunge N, Murata K, Sakaguchi K (1982) J Bacteriol 151:462–464
Hay RT, Stow ND, McDougall IM (1984) J Mol Biol 175:493–510
Hinnen JB, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
Hishinuma F, Nakamura K, Hirai K, Nishizawa R, Gunge N, Maeda T (1984) Nucleic Acids Res 12:7581–7597
Hsiao C, Carbon J (1979) Proc Natl Acad Sci USA 76:3829–3833
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
Kikuchi Y, Hirai K, Hishinuma F (1984) Nucleic Acids Res 12:5685–5692
de Louvencourt L, Fukuhara H, Heslot H, Wesolowski M (1983) J Bacteriol 154:737–742
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Niwa O, Sakaguchi K, Gunge N (1981) J Bacteriol 148:988–990
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sherman F, Fink GR, Hicks JB (1983) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Stinchcomb D, Struhl K, Davis RW (1979) Nature 282:39–43
Sreekrishna K, Webster TD, Dickson RC (1984) Gene 28:73–81
Wesolowski M, Algeri A, Fukuhara H (1982a) Curr Genet 5:205–208
Wesolowski M, Alferi A, Goffrini P, Fukuhara H (1982b) Curr Genet 5:191–197
Wesolowski M, Dumazert P, Fukuhara H (1982c) Curr Genet 5:199–203
Zakian VA, Scott JF (1982) Mol Cell Biol 2:221–232
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Fujimura, Ha., Hishinuma, F. & Gunge, N. Terminal segment of Kluyveromyces lactis linear DNA plasmid pGKL2 supports autonomou sreplication of hybrid plasmids in Saccharomyces cerevisiae . Curr Genet 12, 99–104 (1987). https://doi.org/10.1007/BF00434663
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DOI: https://doi.org/10.1007/BF00434663