Abstract
The benefits of vitamin D3 on fracture healing have been commonly demonstrated in vitamin-D3-depleted animal models. The aim of this study was to examine the effects of a single high dose of vitamin D3 on fracture healing in a healthy animal model, which has not been previously reported. Twenty healthy young adult guinea pigs were randomly divided into two groups as ‘control’ and ‘vitamin D’, and their right tibias were fractured with digital manipulation. Guinea pigs in vitamin D group were injected intramuscularly with 50000 IU/kg of vitamin D3. The animals were killed at 7, 14, 21 and 28 days following fracture. Ultrastructural analysis of the harvested tibias revealed that a single high dose of vitamin D3 stimulated fracture healing. The observed effects at the fracture zone in a healthy animal model included advancement of blood supply, acceleration of synthesis and organization of collagen fibres, acceleration of the proliferation and differentiation of osteoprogenitor cells, and activation of the mineralization of the matrix.
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References
Alkalay D, Shany S, Dekel S (1989) Serum and bone vitamin D metabolites in elective patients and patients after fracture. J Bone Joint Surg [Br] 71b:85–87
Behrman RE, Kliegman RM, Nelson WE, Vaughan VC III (1992) Nelson textbook of pediatrics. W.B. Saunders, Philadelphia
Beresford JN, Gallagher JA, Russell RGG (1986) 1,25-Dihydroxyvitamin D3 and human bone-derived cells in vitro: effects on alkaline phosphatase, type I collagen and proliferation. Endocrinology 119:1776–1785
Brighton CT, Hunt RM (1991) Early histological and ultrastructural changes in medullary fracture callus. J Bone Joint Surg [Am] 73a:832–847
Brumbaugh PF, Speer DP, Pitt MJ (1982) 1α,25-Dihydroxyvitamin D3; a metabolite of vitamin D that promotes bone repair. Am J Pathol 106:171–179
Buckwalter JA, Cruess RL (1991) Healing of the musculoskeletal tissues. In: Rockwood CA Jr, Green DP, Bucholz RW (eds) Rockwood and Green's fractures in adults. J.B. Lippincott, New York, pp 181–222
Dekel S, Salama R, Edelstein S (1983) The effect of vitamin D and its metabolites on fracture repair in chicks. Clin Sci 65:429–436
Erdoğan D, Çalgüner E, Kadioğlu D, Aky1ldiz N, Atalay N (1991) Ultrastructural study of the bone lesion in chronic otitis media. J Neurol Orthop Med Surg 12:195–198
Hiltunen A, Vuorio E, Aro HT (1993) A standardized experimental fracture in the mouse tibia. J Orthop Res 11:305–312
Hulth A (1989) Current concepts of fracture healing. Clin Orthop 249:265–284
Lidor C, Dekel S, Edelstein S (1987) The metabolism of vitamin D3 during fracture healing in chicks. Endocrinology 120:389–393
Lidor C, Dekel S, Hallel T, Edelstein S (1987) Levels of active metabolites of vitamin D3 in the callus of fracture repair in chicks. J Bone Joint Surg [Br] 69b:132–136
Lindgren JU, Narechania RG, McBeath AA, Lange TA, DeLuca HF (1981) Effects of 1.25 dihydroxyvitamin D3 and calcitonin on fracture healing in adult rats. Clin Orthop 160:304–308
Meller Y, Kestenbaum RS, Mozes M, Mozes G, Yagil R, Shany S (1984) Mineral and endocrine metabolism during fracture healing in dogs. Clin Orthop 187:289–295
Meller Y, Kestenbaum RS, Shany S, Zuili I, Yankowitz N, Giat J, Konforti A, Torok G (1984) Parathyroid hormone, calcitonin, and vitamin D metabolites during normal fracture healing in humans; a preliminary report. Clin Orthop 183:238–245
Meller Y, Kestenbaum RS, Shany S, Galinsky D, Zuili I, Yankovitch N, Giat J, Conforti A, Torok G (1985) Parathormone, calcitonin, and vitamin D metabolites during normal fracture healing in geriatric patients. Clin Orthop 199:272–279
Pike JW (1987) Emerging concepts on the biologic role and mechanism of action of 1,25-dihydroxyvitamin D3. Steroids 49:3–27
Raisz LG, Kream BE (1983) Regulation of bone formation. N Engl J Med 309:29–35
Stern PH (1990) Vitamin D and bone. Kidney Int 38 (Suppl 29):517–521
Walters MR (1992) Newly identified actions of the vitamin D endocrine system. Endocr Rev 13:719–764
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Ömeroğlu, S., Erdogan, D. & Ömeroğlu, H. Effects of single high-dose vitamin D3 on fracture healing. Arch Orthop Trauma Surg 116, 37–40 (1997). https://doi.org/10.1007/BF00434098
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DOI: https://doi.org/10.1007/BF00434098