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An efficient cell-free translation system from Aspergillus nidulans and in vitro translocation of prepro-a-factor across Aspergillus microsomes

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Summary

We describe the preparation of an in vitro translation system from heat shock-treated Aspergillus nidulans, capable of supporting efficient and faithful synthesis of proteins from natural and in vitro transcribed eukaryotic messages. In vitro synthesized prepro-α-factor was translocated across Aspergillus nidulans microsomal membranes in either the homologous A. nidulans or a yeast cell-free system. The translocated prepro-α-factor was protected from digestion by protease and glycosylated to higher MW forms.

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Authors and Affiliations

  1. Allelix Inc. Biopharmaceutical Division, 6850 Goreway Drive, L4V 1P1, Mississauga, Ontario, Canada

    M. Devchand, D. Gwynne, F. P. Buxton & R. W. Davies

Authors
  1. M. Devchand
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  2. D. Gwynne
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  3. F. P. Buxton
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  4. R. W. Davies
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Devchand, M., Gwynne, D., Buxton, F.P. et al. An efficient cell-free translation system from Aspergillus nidulans and in vitro translocation of prepro-a-factor across Aspergillus microsomes. Curr Genet 14, 561–566 (1988). https://doi.org/10.1007/BF00434081

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  • DOI: https://doi.org/10.1007/BF00434081

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