Summary
The RAD50 gene in yeast is required for recombination-repair (i.e., the double strand break repair pathway) in mitosis, and for meiotic recombination and sporulation. Both of these processes are complex and seem likely to require a relatively large number of gene products. In order to help define other genes required for recombination and repair processes in yeast, we have isolated extragenic revertants of rad50-4 which restore the ability to grow in the presence of MMS. Evidence from segregation indicates the extragenic revertants fall into at least five loci. Two of them reduce sporulation and spore viability at high temperature; another mutation confers a spontaneous hyperrec phenotype on mitotic cells. Thus, at least three revertants are candidates for mutations which affect recombination functions.
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References
Baker B, Carpenter A, Esposito RE, Sandler L (1976) Ann Rev Genet 10:53–134
Esposito M, Esposito R (1969) Genetics 61:79–89
Esposito M, Wagstaff J (1981) Mechanisms of mitotic recombination. In: Strathern J, Broach J, Jones E (eds) Molecular biology of the yeast Saccharomyces. Cold Spring Harbor Press, Cold Spring Harbor, NY, pp 341–370
Esposito MS, Esposito RE, Armand M, Halverson HO (1969) J Bacteriol 100:180–186
Esposito RE, Plotkin D, Esposito M (1974) The relationship between genetic recombination and commitment to chromosomal segregation at meiosis. In: Grell R (ed) Mechanisms in recombination. Plenum Press, New York, pp 227–285
Game JC, Mortimer R (1974) Mutat Res 24:281–282
Game JC, Johnston LH, von Borstel RC (1979) Proc Natl Acad Sci 76:4389–4594
Game JC, Zamb TJ, Braun RJ, Resnick M, Poth RM (1980) Genetics 94:51–68
Golin JE, Esposito M (1977) Mol Gen Genet 150:127–135
Gorman JA, Gorman J (1971) Genetics 67:337–349
Haynes RH, Kunz BA (1981) DNA repair and mutagenesis in yeast. In: Strathern J, Broach J, Jones E (eds) Molecular biology of the yeast Saccharomyces. Cold Spring Harbor Press, Cold Spring Harbor, NY, pp 371–414
Ho, KSY (1975) Mutat Res 30:327–335
Hopper AK, Hall BD (1975) Genetics 80:41–60
Hopper AK, Kirsch J, Hall BD (1975) Genetics 80:61–78
Klapholz S, Esposito RE (1980a) Genetics 96:580–597
Klapholz S, Esposito RE (1980b) Genetics 96:598–611
Liebman S, Sherman F (1976) Genetics 81:223–232
Malone RE (1983) Mol Gen Genet 189-405–412
Malone RE, Esposito RE (1981) Mol Cell Bio 1:891–901
Mortimer RK, Schild D (1980) Microbiol Rev 44:519–571
Resnick M (1978) The importance of DNA double strand break in yeast. In: Hanawalt PC (ed) DNA repair mechanisms. Academic Press, New York, pp 417–424
Resnick M, Martin P (1976) Mol Gen Genet 143:119–129
Rothstein RJ, Esposito RE, Esposito MS (1977) Genetics 85:35–45
Saeki T, Machida I, Nakai S (1980) Mutat Res 73:251–265
Sherman F (1981) Suppression in yeast. In: Strathern J, Broach J, Jones E (eds) Molecular biology of the yeast Saccharomyces. Cold Spring Harbor Press, Cold Spring Harbor, NY, pp 463–486
Sherman F, Roman H (1963) Genetics 48:255–261
Singh A, Manney TR (1974) Genetics 77:661–674
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Malone, R.E., Jordan, K. & Wardman, W. Extragenic revertants of rad50, a yeast mutation causing defects in recombination and repair. Curr Genet 9, 453–461 (1985). https://doi.org/10.1007/BF00434050
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DOI: https://doi.org/10.1007/BF00434050