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Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae

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Summary

A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes for the sup1 structural gene (chromosome IIR).

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Breining, P., Surguchov, A.P. & Piepersberg, W. Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae . Curr Genet 8, 467–470 (1984). https://doi.org/10.1007/BF00433913

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  • DOI: https://doi.org/10.1007/BF00433913

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