Current Genetics

, Volume 8, Issue 6, pp 399–405 | Cite as

Transmission and recombination of chloroplast genes in asexual crosses of Chlamydomonas reinhardii

I. Flagellar agglutination prior to fusion does not promote uniparental inheritance or affect recombinant frequencies
  • R. E. Galloway
  • L. R. Holden
Original Articles


Chlamydomonas reinhardii diploids homozygous for the plus mating-type (mt+) allele were constructed via polyethylene glycol (PEG)-induced cell fusion to investigate the transmission of chloroplast genes.

We used two methods to determine whether the fusion products (PEG diploids) had inherited chloroplast markers uniparentally or biparentally. One method (multiple clone analysis) was found to markedly improve the detection of biparental transmission. With this method the frequency of biparental PEG-induced diploids was comparable to that seen in sexual diploids. Multiple clone analysis also demonstrated that fusion products which showed biparental inheritance were an extremely heterogeneous group.

In some crosses, pre-treatment of one parent with isolated flagella from cells of the opposite mating type was used. This pre-treatment has been reported to promote chloroplast gene transmission from the mt+ parent in diploids produced by PEG fusion (Adams 1982). In our crosses flagellar pretreatment had no significant effect on chloroplast gene transmission or recombination frequences. A significant bias was found for the date a cross was performed, but neither parent preferentially transmitted its alleles in all crosses. This indicates the necessity of comparing data from treated and control crosses done on the same day. We conclude from our data that separate hypotheses are not required to explain biparental inheritance in sexual versus PEG-induced diploids. The absence of biased parental allelic ratios with PEG-induced biparental diploid clones underscores their usefulness for the study of biparental gene transmission.

Key words

Non-Mendehan genes Diploids Polyethylene glycol Biparental inheritance 


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  1. Adams GMW (1978) Plasmid 1:522–535Google Scholar
  2. Adams GMW (1982) Curr Genet 5:1–3Google Scholar
  3. Birky CW, VanWinkle-Swift KP, Sears BB, Boynton JE, Gillham NW (1981) Plasmid 6:173–192Google Scholar
  4. Bolen PL, Grant DM, Swinton D, Boynton JE, Gillham NW (1982) Cell 28:335–343Google Scholar
  5. Bradley JV (1968) Distribution-Free Statistical Tests. PrenticeHall, Englewood Cliffs, p 388Google Scholar
  6. Chiang KJ, Sueoka N (1967) J Cell Physiol 70:89–112Google Scholar
  7. Ebersold WT (1967) Science 157:447–449Google Scholar
  8. Gillham NW (1963) Nature 200:294Google Scholar
  9. Gillham NW (1969) Amer Naturalist 103:355–388Google Scholar
  10. Gillham NW (1978) Organelle Heredity. Raven Press, New YorkGoogle Scholar
  11. Gillham NW, Boynton JE, Lee RW (1974) Genetics 78:439–457Google Scholar
  12. Goodenough UW, Weiss RL (1975) J Cell Biol 67:623–637Google Scholar
  13. Matagne RF (1981) Curr Genet 3:31–36Google Scholar
  14. Matagne RF, Hermesse M-P (1980) Curr Genet 1:127–131Google Scholar
  15. Matagne RF, Hermesse M-P (1981) Genetics 99:371–381Google Scholar
  16. Mesland D, Hoffman JL, Caligor E, Goodenough UW (1980) J Cell Biol 84:599–667Google Scholar
  17. Mets L (1980) Curr Genet 2:131–138Google Scholar
  18. Sager R, Grabowy C (1983) Proc Natl Acad Sci USA 80:3025–3029Google Scholar
  19. Sager R, Lane D (1972) Proc Natl Acad Sci USA 69:2410–2413Google Scholar
  20. Schlosser UG, Sachs H, Robinson DG (1976) Protoplasma 88:51–64Google Scholar
  21. Sears BB, Boynton JE, Gillham NW (1980) Genetics 96:95–114Google Scholar
  22. Surzycki S (1971) Synchronously grown cultures of Chlamydomonas reinhardii. In: San Pietro A (ed) Methods in enzymology, vol 23. Academic Press, New York, p 67Google Scholar
  23. Van Winkle-Swift KP (1776) Dissertation, Duke University, Durham,Google Scholar
  24. VanWinkle-Swift KP (1980) Curr Genet 1:113–125Google Scholar
  25. VanWinkle-Swift KP, Birky CW (1978) Mol Gen Genet 166:173–209Google Scholar
  26. Whiteway MS, Lee RW (1977) Mol Gen Genet 157:11–15Google Scholar
  27. Witman GB, Carlson K, Berliner J, Rosenbaum JL (1972) J Cell Biol 54:507–539Google Scholar
  28. Wurtz EA, Boynton JE, Gillham NW (1977) Proc Natl Acad Sci USA 74:4552–4556Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • R. E. Galloway
    • 1
  • L. R. Holden
    • 2
  1. 1.Department of BiologyWashington UniversitySt. Louis
  2. 2.Research DivisionMonsanto Agricultural Products CompanySt. LouisUSA

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