Summary
The fraction of mutants measured in a population of ultraviolet (UV) irradiated bacteriophage ϕX174 is higher when the mutants are assayed on host cells which have been irradiated with a small dose of UV than when unirradiated cells are used for assay. Evidence is presented that the UV-induced processes in the bacteria which are responsible for the increased number of mutants introduce mutations prior to the replication of the double-stranded replicative form DNA to which the parental single-stranded ϕX174 DNA is converted after infecting the host cell. These processes also result in an enhanced fraction of “spontaneous” mutants in unirradiated populations of ϕX174. Furthermore, it is shown that the spectrum of mutants in unirradiated as well as in irradiated ϕX174 differs for assay of mutants on unirradiated and UV-irradiated bacteria. The transition A→G is observed less frequently when the phage is assayed on irradiated cells. Neither UV-induced nor “spontaneous” mutation frequencies were enhanced when the UV-irradiated host bacterium carried a recA mutation.
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Communicated by B. A. Bridges
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Bleichrodt, J.F., Verheij, W.S.D. Mutagenesis by ultraviolet radiation in bacteriophage ϕX174: on the mutation stimulating processes induced by ultraviolet radiation in the host bacterium. Molec. Gen. Genet. 135, 19–27 (1974). https://doi.org/10.1007/BF00433897
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DOI: https://doi.org/10.1007/BF00433897