Molecular and General Genetics MGG

, Volume 123, Issue 4, pp 333–346 | Cite as

Temperature sensitive mutants affecting the activity and regulation of the acetamidase of Aspergillus nidulans

  • Pamela Dunsmuir
  • M. J. Hynes
Article

Summary

Mutants of Aspergillus nidulans with temperature sensitive growth on various amides have been isolated. Three of these mutants have a lesion in the amdS gene and their properties indicate that this is the structural gene for the acetamidase enzyme. In addition one of these mutants appears to be temperature sensitive for assembly of enzyme sub-units. The fourth mutant has a lesion in the amdR gene and, while producing a normal enzyme, is temperature sensitive for synthesis of the acetamidase. The properties of these mutants provide support for a model in which amdR codes for a protein which acts positively to activate synthesis of the acetamidase. A discussion of the present knowledge concerning acetamidase regulation is presented.

Keywords

Enzyme Amide Aspergillus Structural Gene Present Knowledge 

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Pamela Dunsmuir
    • 1
  • M. J. Hynes
    • 1
  1. 1.Department of GeneticsLa Trobe UniversityBundooraAustralia

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