Serum enzyme variations in men during an exhaustive “square-wave” endurance exercise test

  • Manuel Gimenez
  • Michel Florentz
Original Articles


The present study was designed to test if both the intensity and duration of the 45-min Square-Wave Endurance Exercise Test (SWEET) would produce changes in serum enzyme activities. Nine men, four sedentary (S) and five athletes (A), performed \(\dot V_{{\text{O}}_{\text{2}} }\) max and SWEET, at their Maximal Intensity of Endurance (MIE45) as defined by maximal heart rate and the impossibility of maintaining MIE 45+5% for 45 min. Arterial blood was sampled at rest (R), exercise (Ex) (45th min) and during recovery (15th min) for measurements of levels of Haemoglobin (Hb), Haematocrit (Hct), pH and seven serum enzymes: Creatine kinase (CPK), Hexose-phosphate isomerase (PHI), Aldolase (ALD), Lactate dehydrogenase (LDH), Malate dehydrogenase (MDH), Aspartate amino-transferase (ASAT or GOT), and Alanine aminotransferase (ALAT or GPT). Five enzymes increased significantly during exercise (MIE45), the δ% (Ex — R/R) increases were as follows: PHI (72%), MDH (28%), LDH (21%), CPK (17%), and GOT (13.5%), whilst only a 10% increase was observed for Hct and Hb and there was no significant change in the arterial pH. There was no correlation between the δ% of Hb, Hct, pH, and the results for the enzymes. Thus, it does not seem that haemoconcentration and arterial blood acidosis which occur during exercise are only at the origin of the observed increases in enzymes. A difference between “sedentary” and “athletes” subjects was found at rest and exercise (δ%=A−S/S) for CPK (R=222%; Ex=235%), GOT (R=90%; Ex=75%) and ALD (R=99%; Ex=54%). These results suggest that the MIE45, by measured increases in enzymatic activity, seems to require great muscular effort.

Key words

Serum enzymes (CPK, PHI, MDH, LDH, GOT, GPT, ALD) Arterial pH Haemoglobin Cycle-ergometer Sedentary men Athletes 


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Manuel Gimenez
    • 1
  • Michel Florentz
    • 1
  1. 1.Laboratory of Exercise Physiology, Unité 14 of the Institut National de la Santé et de la Recherche Médicale (INSERM)Centre Hospitalier Universitaire de BraboisVandoeuvre-lés-NancyFrance

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