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Lipid metabolism of the arterial wall in Thromboangiitis obliterans (Buerger's disease)

Virchows Archiv A volume 369, pages 123–130 (1975)Cite this article

Summary

The uptake and incorporation of [I14-C] oleic acid by diseased arterial intima removed by thrombendarteriectomy in 3 patients with Thromboangiitis obliterans (TAO) was studied.

The diagnosis of TAO had been established by clinical, angiographic and histological criteria.

The uptake and distribution of the label was found very similar in TAO and normal intima and differed considerably from atherosclerotic intima, from fatty streaks as well as from fibro-fatty lesions.

In fatty streak lesions the incorporation of [I14-C] oleic acid into phospholipid, triglyceride and cholesterol ester was significantly increased compared to TAO, normal intima and umbilical artery.

In TAO the distribution of labelled lipids between subcellular fractions of the arterial intima was also studied and, as in normal intima, most of the cholesterol ester were found membrane-bound whereas in fibro-fatty lesions the bulk of the cholesterol ester was present in the lipid skin fraction.

The incorporation of [I14-C] oleic acid into different phospholipids was highest in atherosclerotic intima while no significant differences were found between normal intima and TAO.

These data suggest a different pathogenesis of TAO and atherosclerosis.

Zusammenfassung

Aufnahme und Einbau von [I14-C] Ölsäure in die thrombangiitisch veränderte Intima wurde an Gefäßzylindern untersucht, die bei 3 Patienten im Rahmen einer Thrombendarterektomie entnommen worden waren. Bei diesen Patienten war die Diagnose Thrombangiitis obliterans (TAO) auf Grund klinischer, angiographischer und histologischer Befunde gestellt worden.

Der Einbau des Isotops in die verschiedenen Lipide der Gefäßwand war bei TAO und normaler Intima sehr ähnlich und unterschied sich deutlich von dem Einbau in atherosklerotische Intima und zwar sowohl in Fettstreifen als auch in Atherome.

Bei Fettstreifen war der Einbau von [I14-C] Ölsäure in Phospholipide, Triglyzeride und Cholesterin-Ester gegenüber der TAO, normaler Intima und Nabelschnurarterien signifikant erhöht. Die Verteilung der markierten Lipide auf verschiedene subcelluläre Fraktionen der Intima war in dem untersuchten thrombangiitischen Gewebe jener der normalen Intima vergleichbar: Die Cholesterinveresterung fand ganz überwiegend membrangebunden statt, während bei Atheromen die markierten Cholesterin-Ester vor allem in der überstehenden Fettschicht nachweisbar waren.

Der Einbau des Isotops in verschiedene Phospholipide war bei atherosklerotischer Intima wesentlich höher als in den anderen untersuchten Geweben. Zwischen normaler und thromboangiitischer Intima fanden sich keine signifikanten Unterschiede in der Verteilung der markierten Phospholipide.

Diese Befunde legen eine eigenständige, von der Atherosklerose unabhängige Pathogenese der Thromboangiitis obliterans nahe.

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Affiliations

  1. Medizinische Universitätsklinik, Heidelberg

    A. K. Horsch

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  1. A. K. Horsch
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Additional information

The studies were supported by the German Research Foundation within the SFB 90 „Cardiovasculäres System”.

We are grateful to Dr. K. Laubach, Chirurgische Universitäts-Klinik Heidelberg, and to Dr. S. K. Horsoh, Chirurgische Universitätsklinik Köln, for provision of surgery specimens.

The excellent technical assistance of Mrs. I. Leyendecker is also gratefully acknowledged.

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Horsch, A.K. Lipid metabolism of the arterial wall in Thromboangiitis obliterans (Buerger's disease). Virchows Arch. A Path. Anat. and Histol. 369, 123–130 (1975). https://doi.org/10.1007/BF00433238

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  • DOI: https://doi.org/10.1007/BF00433238

Key words

  • Peripheral vascular disease
  • Atherosclerosis
  • Lipid synthesis
  • Cholesterol ester
  • Phospholipid
  • Subcellular fractions
  • [I14-C] Oleic acid