Molecular and General Genetics MGG

, Volume 107, Issue 1, pp 50–57 | Cite as

In vivo repair of UV-irradiation damage of single stranded DNA phage ΦX 174

  • B. Datta
  • R. K. Poddar


When E. coli C cells, infected with UV irradiated ΦX 174, were allowed to grow in liquid tris-glucose medium at 37° C with aeration, the UV damage of the single stranded (ss) DNA could be repaired to some extent. Such repair was not possible if the irradiated phage were plated immediately on E. coli C in the usual double layer agar method, or if the infected complexes were initially exposed to 0.02 M KCN for 15 min before they were allowed to grow in tris-glucose medium as before. Our results indicate that in order to be repaired, ss DNA containing UV damage must be able to convert itself to a closed circular double stranded replicative form (RF) within the host cells escaping prior scission. The whole process of repair was found to be dependent on protein synthesis in the infected complexes.


Agar Host Cell Aeration Double Layer Scission 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adams, M. H.: Bacteriophage. New York: Interscience Publishers Inc. 1959.Google Scholar
  2. Boyce, R. P., Howard-Flanders, P.: Release of ultraviolet light induced dimers from DNA in Escherichia coli K 12. Proc. Nat. Acad. Sci. (Wash.) 31, 293–300 (1964).Google Scholar
  3. Burton, A., Sinsheimer, R. L.: The process of infection with bacteriophage ΦX 174, VII. Ultracentrifugal analysis of the replicative form. J. molec. Biol. 14, 327–347 (1965).Google Scholar
  4. Cairns, J., Denhardt, D. T.: Effect of cyanide and carbon monooxide on the replication of bacterial DNA in vivo. J. molec. Biol. 36, 335–342 (1968).Google Scholar
  5. Fiers, W., Sinsheimer, R. L.: The structure of the DNA of bacteriophage ΦX 174. III. Ultracentrifugal evidence for a ring structure. J. molec. Biol. 5, 424–434 (1962).Google Scholar
  6. Fujimura, R., Kaesberg, P.: The adsorption of bacteriophage ΦX 174 to its host. Biophys. J. 2, 433–449 (1960).Google Scholar
  7. Goulian, M., Kornberg, A., Sinsheimer, R. L.: Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage ΦX 174 DNA. Proc. nat. Acad. Sci. (Wash.) 58, 2321 (1967).Google Scholar
  8. Guthrie, S., Sinsheimer, R. L.: Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage ΦX 174. Biochim. biophys. Acta (Amst.) 72, 290–297 (1963).Google Scholar
  9. Jansz, H. S., Pouwels, P. H., Rotterdam, J. van: Sensitivity to ultraviolet light of single and double stranded DNA. Biochim biophys. Acta (Amst.) 76, 655–657 (1963).Google Scholar
  10. Ono, J., Shimazu, Y.: Ultraviolet reactivation of a bacteriophage containing a single stranded deoxyribonucleic acid as a genetic element. Virology 29, 295–302 (1966).Google Scholar
  11. ——: In vivo cleavage of a circular single stranded DNA of bacteriophage ΦR irradiated with ultraviolet light. J. molec. Biol. 24, 491–495 (1967).Google Scholar
  12. Pettijohn, D., Hanawalt, P.: Evidence for repair replication of ultraviolet damaged DNA in bacteria. J. molec. Biol. 9, 395–410 (1964).Google Scholar
  13. Pouwels, P. H., Jansz, H. S., Rotterdam, J. van, Cohen, J. A.: Structure of the replicative form of a bacteriophage ΦX 173: Physiochemical studies. Biochim. biophys. Acta (Amst.) 119, 289–300 (1966).Google Scholar
  14. Rörsch, A., Delman, A. E., Cohen, J. A.: The gene controlled radiation sensitivity of Escherichia coli. Biochem. biophys. Acta. (Amst.) 68, 263–270 (1963).Google Scholar
  15. Rupp, W. D., Howard-Flanders, P.: Discontinuities in the DNA synthesized in an excision defective strain of Escherichia coli following ultraviolet irradiation. J. molec. Biol. 31, 291–304 (1968).Google Scholar
  16. Setlow, R. B., Carrier, W. L.: The disappearance of thymine dimers from DNA: an error correcting mechanism. Proc. nat. Acad. Sci. (Wash.) 51, 226–231 (1964).Google Scholar
  17. Sinsheimer, R. L., Starman, B., Nagler, C., Guthrie, S.: The process of infection with bacteriophage ΦX 174. I. Evidence for a “Replicative form”. J. molec. Biol. 4, 142–160 (1962).Google Scholar
  18. Tessman, E. S., Ozaki, T.: The interaction of phage S 13 with ultraviolet-irradiated host cells and properties of the ultraviolet-irradiated phage. Virology 12, 431–449 (1960).Google Scholar
  19. Yarus, M., Sinsheimer, R. L.: The UV resistance of double-stranded ΦX 174 DNA. J. molec. Biol. 8, 614–615 (1964).Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • B. Datta
    • 1
  • R. K. Poddar
    • 1
  1. 1.Biophysics LaboratorySaha Institute of Nuclear PhysicsCalcutta-37India

Personalised recommendations