Abstract
5-Hydroxytryptamine (5-HT) uptake was studied by using blood platelets from 13 patients with endogenous depression (Hamilton rating scale 33±7) and 13 healthy volunteers. An improved method with a short incubation time and low substrate concentration was used, and the incubation was performed in Krebs-Henscleit buffer (pH 7.4) at 37° C. A clear difference in 5-HT uptake by blood platelets was noted: The V max of the reaction in patients was 39, and in controls 71 pmol per 2×107 platelets in 5 min. There was no significant difference in the K m. After a 4-week treatment with imipramine, a competitive inhibition of 5-HT uptake with an increased K m was seen; after a similar treatment with amoxapine there was little change in 5-HT uptake. Amoxapine was inferior to imipramine as an inhibitor of 5-HT uptake, also in vitro. There was no difference in clinical recovery in these treatment groups. These results may be of importance so as to understand the potential biological differences between depressed patients and normal persons.
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References
Airaksinen, E. M.: Platelet-rich plasma 5-hydroxytryptamine, urinary 5-hydroxyindoleactic acid and tryptophan ingestion in mongols. J. Ment. Defic. Res. 15, 244–256 (1971)
Aminoff, M. J., Trenchard, A., Turner, P., Wood, W. G., Hills, M.: Plasma uptake of dopamine and 5-hydroxytryptamine and plasma-catecholamine levels in patients with Huntington's chorea. Lancet II 1974, 1115–1116
Bayer, S. M., McCoy, E. E.: Serotonin uptake and adenine nucleotide metabolism in platelets of Down's syndrome patients: A comparison of the serotonin and ATP content in platelets from subjects with Down's syndrome. Biochem. Med. 9, 225–232 (1974)
Beck, A. R., Beck, R. W.: Screening depressed patients in family practice. A rapid technique. Postgrad. Med. 52, 81–85 (1972)
Born, G. V. R., Gillson, R. E.: Studies on the uptake of 5-hydroxytryptamine by blood platelets. J. Physiol. (Lond.) 146, 472–491 (1959)
Boullin, D. J., O'Brien, R. A.: Abnormalities of 5-hydroxytryptamine uptake and binding by blood platelets from children with Down's syndrome. J. Physiol. (Lond.) 212, 287–297 (1971)
Brase, D. A., Loh, H. H.: Comparison of the inhibition of 5-hydroxytryptamine uptake by methadone and its congeners in human platelets. Biochem. Pharmacol. 25, 1684–1686 (1976)
Carlsson, A., Fuxe, K., Ungerstedt, U.: The effect of imipramine of central 5-hydroxytryptamine neurons. J. Pharm. Pharmacol. 20, 150–151 (1968)
Coppen, A., Ghose, K., Swade, C., Wood, K.: Effect of mianserin hydrochloride on peripheral uptake mechanisms for noradrenaline and 5-hydroxytryptamine in man. Br. J. Clin. Pharmacol. 5, 138–178 (1978a)
Coppen, A., Swade, C., Wood, K.: Platelet 5-hydroxytryptamine accumulation in depressive illness. Clin. Chim. Acta 87, 165–168 (1978b)
Drummond, A. H.: Interactions of blood platelets with biogenic amines: Uptake, stimulation and receptor binding. In: Platelets in biology and pathology, J. L. Gordon, ed., pp. 203–239. Amsterdam: North Holland 1976
Glassman, A. H., Perel, J. M.: Tricyclic blood levels and clinical outcome: A review of the art. In: Psychopharmacology: A generation of progress, M. A. Lipton, A. DiMascio, K. F. Killam, eds., pp. 917–922. New York: Raven 1978
Hallstrom, C. O. S., Pare, C. M. B., Rees, W. L., Trenchard, A., Turner, P.: Platelet uptake of 5-hydroxytryptamine and dopamine in depression. Postgrad. Med. J. (Suppl.) 52, 40–44 (1976)
Hamilton, M.: A rating scale for depression. J. Neurol. Neurosurg. Psychiatry 23, 36–62 (1960)
Hammer, W., Sjöqvist, F.: Plasma levels of monomethylated tricyclic antidepressants during treatment with imipramine-like compounds. Life Sci. 6, 1896–1903 (1967)
Jerome, H., Kamoun, P.: Anomalie de la captation de la sérotonine par les plaquettes sanguines des sujets trisomiques 21. Etude charactéristiques générales de la captation. Bull. Soc. Chim. Biol. 50, 907–921 (1968)
Krebs, H. A.: Body size and tissue respiration. Biochim. Biophys. Acta 4, 249–269 (1950)
Lineweaver, H., Burk, D.: The determination of enzyme dissociation constants. J. Am. Chem. Soc. 56, 658–666 (1934)
Lingjaerde, O., Jr.: Uptake of serotonin in blood platelets: Dependence on sodium and chloride, and inhibition by chloride. FEBS Letters 3, 103–106 (1969)
Lingjaerde, O.: Effect of doxepine on uptake and efflux of serotonin in human blood platelets in vitro. Psychopharmacologia 47, 183–186 (1976)
Lott, I. T., Chase, T. N., Murphy, D. L.: Down's syndrome: Transport, storage, and metabolism of serotonin in blood platelets. Pediatr. Res. 6, 730–735 (1972)
Lucas, A. R., Warner, K., Gottlieb, J. S.: Biological studies in childhood schizophrenia: Serotonin uptake by platelets. Biol. Psychiatry 3, 123–128 (1971)
Marshall, E. F., Stirling, G. S., Tait, A. C., Todrick, A.: The effect of iproniazid and imipramine on the blood platelet 5-hydroxytryptamine level in man. Br. J. Pharmacol. 15, 35–41 (1960)
McCoy, E. E., Rostafinsky, M. J., Fishburn, C.: The concentration of serotonin by platelets in Down's syndrome. J. Ment. Defic. Res. 12, 18–21 (1968)
McLean, D. R., Nihei, T.: Uptake of dopamine and 5-hydroxytryptamine by platelets from patients with Huntington's chorea. Lancet I 1977 249–250
Paasonen, M. K.: Platelet 5-hydroxytryptamine as a model in pharmacology. Ann. Med. Exp. Biol. Fenn. 46, 416–422 (1968)
Paasonen, M. K.: Blood platelets as a model for aminergic neurons. In: Proceedings of the fifth international congress on pharmacology, Vol. 4, G. H. Acheson, ed., pp. 328–342. Basel: Karger 1973
Pletscher, A.: Platelets as models for monoaminergic neurons. In: Essays in neurochemistry and neuropharmacology, Vol. 3, M. B. H. Youdim, ed., pp. 49–101. London: Wiley 1978
Shaw, D. M., MacSweeney, D. A., Woolcock, N., Bevan-Jones, A. B.: Uptake and release of 14C-5-hydroxytryptamine by platelets in affective illness. J. Neurol. Neurosurg. Psychiatry 34, 224–225 (1971)
Sneddon, J. M.: Sodium-dependent accumulation of 5-hydroxytryptamine by rat blood platelets. Br. J. Pharmacol. 37, 680–688 (1969)
Sneddon, J. M.: Blood platelets as a model for monoamine-containing neurones. Prog. Neurobiol. 1, 151–158 (1973)
Solatunturi, E., Tuomisto, J.: Uptake of 5-hydroxytryptamine and histamine by intracellular particles of rabbit blood platelets. Ann. Med. Exp. Biol. Fenn. 46, 447–452 (1968)
Stahl, S. M.: The human platelet: A diagnostic and research tool for the study of biogenic amines in psychiatric and neurologic disorders. Arch. Gen. Psychiatry 34, 509–516 (1977)
Stahl, S. M., Meltzer, H. Y.: A kinetic and pharmacologic analysis of 5-hydroxytryptamine transport by human platelets and platelet storage granules: Comparison with central serotonergic neurons. J. Pharmacol. Exp. Ther. 205, 118–132 (1978)
Tuomisto, J.: A new modification for studying 5-HT uptake by blood platelets: A re-evaluation of tricyclic antidepressants as uptake inhibitors. J. Pharm. Pharmacol. 26, 92–100 (1974)
Tuomisto, J., Tukiainen, E.: Decreased uptake of 5-hydroxytryptamine in blood platelets from depressed patients. Nature 262, 596–598 (1976)
Tuomisto, J., Walaszek, E. J., Smissman, E. E., Pazdernik, T. L.: Decalin analogs of phenethylamines as inhibitors of amine uptake by rabbit platelets: Uptake of 5-hydroxytryptamine. J. Pharm. Sci. 63, 1714–1718 (1974)
Wielosz, M., De Gaetano, G., Garattini, S.: Improved method for evaluating the inhibition of (14C)5-hydroxytryptamine uptake by rat platelets. J. Pharm. Pharmacol. 28, 717–718 (1976a)
Wielosz, M., Salmona, M., De Gaetano, G., Garattini, S.: Uptake of 14C-5-hydroxytryptamine by human and rat platelets and its pharmacological inhibition. Naunyn Schmiedebergs Arch. Pharmacol. 296, 59–65 (1976b)
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Tuomisto, J., Tukiainen, E. & Ahlfors, U.G. Decreased uptake of 5-hydroxytryptamine in blood platelets from patients with endogenous depression. Psychopharmacology 65, 141–147 (1979). https://doi.org/10.1007/BF00433040
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DOI: https://doi.org/10.1007/BF00433040