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Psychopharmacology

, Volume 79, Issue 1, pp 45–48 | Cite as

Effect of naloxone and amphetamine on acquisition and memory consolidation of active avoidance responses in rats

  • Susana Fulginiti
  • Liliana M. Cancela
Original Investigations

Abstract

Pretraining IP injection of naloxone (0.3 mg/kg) or amphetamine (2 mg/kg) enhanced performance during acquisition, but did not improve retention of active avoidance responses in rats. Naloxone (0.1 or 3 mg/kg) had no effect on acquisition or on retention. The combination of naloxone (0.3 mg/kg) plus amphetamine (2 mg/kg) did not produce the facilitation observed when each of the two drugs was administered alone. Pretreatment with the higher dose of naloxone (3 mg/kg) blocked the facilitative effect of amphetamine on acquisition. Post-training administration of naloxone (0.3 mg/kg) or amphetamine (2 mg/kg) improved retention. Naloxone (0.1 or 3 mg/kg) had no effect. When naloxone and amphetamine were combined, at respective doses of 0.3 mg/kg and 2 mg/kg, the improvement did not occur, i.e., the higher dose of naloxone prevented the facilitative effect of amphetamine. In addition, an ineffective dose of amphetamine (0.5 mg/kg), given either pre-or post-training together with the lower dose of naloxone (0.1 mg/kg), produced a significant enhancement of acquisition or consolidation, respectively. The results are consistent with the possibility that naloxone might exert its facilitative action on acquisition and memory consolidation through the release of catecholaminergic systems from inhibitory influences of opioids.

Key words

Naloxone Amphetamine Active avoidance conditioning Post-training treatment Pretraining treatment retention Acquisition Learning Memory consolidation Rat 

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References

  1. Baghat B (1970) Effects of chronic administration of nicotine on storage and synthesis of noradrenaline in rat brain. Br J Pharmacol 38:86–92Google Scholar
  2. Bliss CI (1967) Statistics in biology, vol 1. McGraw Hill, New YorkGoogle Scholar
  3. Bovet D, Gatti GL, Franck M (1961) An automatic device for the study of conditioned escape reaction in the rat. Sci Rep Ist Super Sanita 1: 127–138Google Scholar
  4. Carr LA, Moore KE (1969) Norepinephrine: Release from brain by d-amphetamine in vivo. Science 164:322–323Google Scholar
  5. Dettmar PW, Cowan A, Walter DS (1978) Naloxone antagonizes behavioural effects of d-amphetamine in mice and rats. Neuropharmacology 17:1041–1044Google Scholar
  6. DeWied D, Bohus B, Van Ree JM, Urban I (1978a) Behavioral and electrophysiological effects of peptides related to lipotropin (β-LPH). J Pharmacol Exp Ther 204:570–580Google Scholar
  7. DeWied D, Kóvács GL, Bohus B, Van Ree JM, Greven HM (1978b) Neuroleptic activity of the neuroleptic β-LPH62–77 (des-tyr1-γ-endorphin, DTγε). Eur J Pharmacol 49:427–436Google Scholar
  8. Doty B, Doty L (1966) Facilitation effects of amphetamine on avoidance conditioning in relation to age and problem difficulty. Psychopharmacologia 9:234–241Google Scholar
  9. Evangelista AM, Izquierdo I (1971) The effect of pre-and post-trial amphetamine injections on avoidance responses of rats. Psychopharmacologia 20:42–47Google Scholar
  10. Fulginiti S, Molina VA, Orsingher OA (1976) Inhibition of catecholamine biosynthesis and memory processes. Psychopharmacology 51:65–69Google Scholar
  11. Fulginiti S, Orsingher OA (1971) Effects of learning, amphetamine and nicotine on the level and synthesis of brain noradrenaline in rats. Arch Int Pharmacodyn Ther 190:291–297Google Scholar
  12. Gallagher M, Kapp BS (1978) Manipulation of opiate activity in the amigdala alters memory processes. Life Sci 23:1973–1978Google Scholar
  13. Gold PE, McGaugh SL (1975) A singletrace, two-processes view of memory storage processes. In: Deusch D, Deusch JA (eds) Shortterm memory. Academic, New York, pp 356–378Google Scholar
  14. Goldstein A, Gordon TP, Leon SO, Ferd L (1976) On the role of endogenous opioid peptides: Failure of naloxone to influence shock escape threshold in the rat. Life Sci 18:599–604Google Scholar
  15. Göthert M, Pohl IM, Weh King E (1979) Effects of presynaptic modulators on Ca2+-induced noradrenaline release from central noradrenergic neurons. Naunyn-Schmiedeberg's Arch Pharmacol 307:21–27Google Scholar
  16. Holtzman SG (1974) Behavioral effects of separate and combined administration of naloxone and d-amphetamine. J Pharmacol Exp Ther 189:51–59Google Scholar
  17. Izquierdo I (1979) Effect of naloxone and morphine on various forms of memory in the rat: Possible role of endogenous opiate mechanisms in memory consolidation. Psychopharmacology 66:199–203Google Scholar
  18. Izquierdo I (1980) Effect of β-endorphin and naloxone on acquisition, memory and retrieval of shuttle avoidance and habituation learning in rats. Psychopharmacology 69:111–115Google Scholar
  19. Izquierdo I, Graudenz N (1980) Memory facilitation by naloxone is due to release of dopaminergic and β-adrenergic systems from tonic inhibition. Psychopharmacology 67:265–268Google Scholar
  20. Izquierdo I, Paiva ACM, Elisabetsky E (1980) Post-training intraperitoneal administration of leuenkephalin and beta-endorphin causes retrograde amnesia for two different tasks in rats. Behav Neural Biol 28:246–250Google Scholar
  21. Kastin AJ, Scollan EL, King MG, Schally AV, Coy DH (1976) Enkephalin and a potent analog facilitate maze performance after intraperitoneal administration in rats. Pharmacol Biochem Behav 5: 691–695Google Scholar
  22. Kety SS (1970) The biogenic amines in the central nervous system: Their possible roles in arousal, emotion, and learning. In: Schmitt FO (ed) The neurosciences. Rockefeller University Press, New York, pp 324–336Google Scholar
  23. Loh HH, Brase DA, Sampath-Khanna S Mar JB, Leong Way E, Li CH (1976) β-Endorphin in vitro inhibition of striatal dopamine release. Nature 264:567–568Google Scholar
  24. McGaugh JL, Petrinovich L (1965) Effects of drugs on learning and memory. Int Rev Neurobiol 8:139–196Google Scholar
  25. Messing RB, Jensen RA, Martínez Jr JL, Spienler VR, Vasquez BJ, Soumireu-Mourat B, Liang KC, McGaugh JL (1979) Naloxone enhancement of memory. Behav Neural Biol 27:266–275Google Scholar
  26. Morgan WW, Pfeil KA (1979) Mecamylamine blockade of nicotine enhanced noradrenaline turnover in rat brain. Life Sci 24:417–420Google Scholar
  27. Orsingher OA, Fulginiti S (1971) Effects of α-methyl-tyrosine and adrenergic blocking agents on the facilitating action of amphetamine and nicotine on learning in rats. Psychopharmacologia 19:231–240Google Scholar
  28. Pollard H, Llorens-Cortes C, Schwartz JC (1977) Enkephalin receptors on dopaminergic neurones in rat striatum. Nature 268:745–747Google Scholar
  29. Raiteri M, Levi G (1978) Release mechanism for catecholamines and serotonin in synaptosomes. In: Ehrenpreis S, Kopin I (eds) Reviews of neuroscience. Raven, New York, pp 77–130Google Scholar
  30. Rigter H (1978) Attenuation of amnesia in rats by systemically administered enkephalins. Science 200:83–85Google Scholar
  31. Rigter H, Hannan TJ, Messing RB, Martínez Jr JL, Vasquez BJ, Jensen RA, Veliquette J, McGaugh JL (1980) Enkephalins interfere with acquisition of an active avoidance response. Life Sci 26:337–345Google Scholar
  32. Taube HD, Borowski E, Endo T, Starke K (1976) Enkephalin: A potential modulator of noradrenaline release in rat brain. Eur J Pharmacol 38:377–380Google Scholar
  33. Zornetzer SF (1978) Neurotransmitter modulation and memory. A new neuropharmacological phrenology? In: Lipton MA, DiMascio A, Killam KF (eds) Psychopharmacology: A generation of progress. Raven, New York, pp 637–649Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Susana Fulginiti
    • 1
  • Liliana M. Cancela
    • 1
  1. 1.Departamento de Farmacología, Facultad de Ciencias QuímicasUniversidad Nacional de CórdobaCórdobaArgentina

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