Abstract
The action of copper (CuSO4, 5mg/kg, oral) on selected neuropharmacological actions of cannabis resin (CI, oral) was studied on albino rats and mice. Copper potentiated the barbiturate hypnosispotentiating activity of CI in albino rats and mice and had no effect on hypothermic activity in albino rats.
Single doses of copper partially inhibited tolerance to barbiturate hypnosis-potentiation activity and markedly delayed the development of tolerance to hypothermic activity of CI. Oral as well as i.c.v. copper (CuSO4, 0.1 μg) in single dose antagonised the tolerance to hypothermic activity of cannabis or THC for to two weeks. Copper-CI interaction could be antagonised by penicillamine. Zinc (ZnSO4, 5 mg/kg, oral) had an action similar to that of copper in antagonising the development of tolerance to the hypothermic activity of CI, but magnesium (MgSO4, 5 mg/kg, i.p.) was devoid of any such action.
Studies indicate that, although copper has no significant neuropharmacological action, it interacts with CI activity, especially in tolerant rats, in effects on hypothermia. The site of action of copper is possibly the hypothalamus, where it inhibits the processes of tolerance development to CI on the noradrenergic neurone.
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References
Chopra, I. C., Chopra, R. N.: The use of cannabis drugs in India. Bull. Narc. 9, 4–29 (1957)
Costa, E., Neff, N. H.: Estimation of turnover rates to study the metabolic regulation of the steady state level of neuronal monoamines. In: Handbook of neurochemistry, vol. IV, A. Lajtha, ed., p. 45. London: Plenum Press 1970
Dube, K.C.: Manner of intake, different preparations, frequency, dose, etc. I.C.M.R. seminar on long-term effect of cannabis use in India, New Delhi, December 1972
Feldberg, W., Lotti, V.J.: Temperature responses to monoamines and an inhibitor of MAO injected into the cerebral ventricles of rats. Br. J. Pharmacol. 31, 152–161 (1967)
Gaur, D. S., Gupta, L. P.: A study on drug evaluation in ancient India. In: Advances in research in Indian Medicine, K. N. Udupa, G. N. Chaturvedi, and S. N. Chaturvedi, eds., p. 357. Varanasi: B. H. U. Press 1970
Goldstein, M., Joh, T. H., Garvey, T. Q.: Biochemistry 7 [pp. 2724–2730, 1968]. Quoted in: Molinoff, P. B., Axelrod, J.: Biochemistry of catecholamines. Annu. Rev. Biochem. 40, 465–500 (1968)
Kitabchi, A. E., Williams, R. H.: Phenylethanolamine-N-methyl transferase in human adrenal gland. Biochim. Biophys. Acta 178, 181–184 (1969)
Lehninger, A. L.: Role of metal ions in enzyme systems. Physiol. Rev. 30, 393–429 (1950)
Lovenberg, W., Bruckwick, E., Hanbauer, I.: Protein phosphorylation and regulation of catecholamine synthesis. In: Chemical tool in catecholamine research II, O. Almgren, A. Carlsson, and J. Angel, eds., p. 37. Amsterdam: North Holland Publishing Co. 1975
Molinoff, P. B., Nelson, D. L., Orcutt, J. C., Harden, T. K.: The regulation of dopamine-β-hydroxylase activity. In: Chemical tool in catecholamine research II, O. Almgren, A. Carlsson, and J. Angel, eds., p. 81. Amsterdam: North Holland Publishing Co. 1975
Molinoff, P. B., Orcutt, J. C.: Dopamine betahydroxylase and the regulation of catecholamine biosynthesis. Frontiers in catecholamine research, p. 195. London: Pergamon Press 1973
Rajan, K. S., Colburn, R. W., Davis, J. M.: Distribution of metal ions in the subcellular fractions of several rat brain areas. Life Sci 18, 423–432 (1976)
Sen, K. B., Sen, K. A., Sen, K. P.: Copper In: Datta's Materia medica of Hindus. Calcutta: Adi-Ayurveda Machine Press 1922
Singh, P. P., Das, P. K.: A neuro-psychopharmacological study of Canabis indica. Ind. J. Pharmacol. 7, 51–57 (1975)
Singh, P. P., Das, P. K.: Role of catecholamines in the hypothermic activity of cannabis in albino rats. Psychopharmacology (Berl.) 50, 199–204 (1976)
Singh, P. P., Das, P. K.: Tolerance to cannabis in albino rats. Ind. J. Exp. Biol. 15, 280–284 (1977)
Singh, P. P., Das, P. K.: Effect of Cannabis indica on locomotor activity. Ind. J. Ex. Biol. (in press, 1978)
Steinberger, R., Westheimer, F. H.: Metal-ion catalyzed decarboxylation dimethyloxaloacetic acid. J. Am. Chem. Soc. 71, 4158 (1949)
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Singh, P.P., Das, P.K. Studies on the interactions of copper and cannabis. Psychopharmacology 56, 309–316 (1978). https://doi.org/10.1007/BF00432855
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DOI: https://doi.org/10.1007/BF00432855