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Virchows Archiv A

, Volume 381, Issue 3, pp 331–342 | Cite as

Glomerulonephritis induced by high doses of ovalbumin

Studies by electron microscopy, immunofluorescence and immuno-electron microscopy
  • Heikki Helin
  • Immo Rantala
  • Juhani Väisänen
  • Amos Pasternack
Original Articles

Summary

Experimental glomerulonephritis was produced in 16 rabbits by intravenous injections of ovalbumin in high doses (0.1 g/day during the first week, 0.2 g×6/day during the second). The animals were killed on day 14. At that time all animals had 2–4+ proteinuria and a serum C3 level reduced to about 50% of the control level; 11 animals had a significantly raised blood urea level. In all rabbits the antigen had induced severe proliferative glomerulonephritis. Electron microscopy showed that many of the cells accounting for the hypercellularity were monocytes. Surprisingly, electron dense deposits were few and small, mainly on the subendothelial and subepithelial aspects of the glomerular basement membrane. In all the animals ultrastructural immunoperoxidase technique revealed deposits containing ovalbumin, rabbit IgG and C3. With immunofluorescence sparse deposits were occasionally seen. It is concluded that a severe experimental glomerulonephritis can be produced in a state of antigen excess, with the deposition of immune complexes being minimal. Immuno-electron microscopy is essential, however, in detecting even the smallest amounts of deposited immune reactants.

Key words

Ovalbumin Glomerulonephritis Immune complex disease Immune deposits Immuno-electron microscopy 

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Heikki Helin
    • 1
  • Immo Rantala
    • 1
  • Juhani Väisänen
    • 1
  • Amos Pasternack
    • 1
  1. 1.Departments of Biomedical and Clinical SciencesUniversity of TampereTampereFinland

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