, Volume 67, Issue 2, pp 143–146 | Cite as

Effect of 5,7-dihydroxytryptamine on the development of tolerance to ethanol

  • Anh Dûng Lê
  • Jatinder M. Khanna
  • Harold Kalant
  • A. Eugene LeBlanc
Original Investigations


5,7-Dihydroxytryptamine (5,7-DHT) or the vehicle was administered once into both lateral ventricles of the rat. Desmethylimipramine (DMI) was administered IP prior to the intraventricular injection of 5,7-DHT to prevent the destruction of norepinephrine (NE) terminals. Following recovery from surgery, ethanol (5 g/kg, PO) or isocaloric sucrose was given daily for 25 days. Tests at 5-day intervals showed that chronic ethanol treatment produced tolerance to the motor impairment on the moving belt test and to hypothermic effects of ethanol. The 5,7-DHT treatment did not alter either the motor impairment or hypothermia produced by the initial dose of ethanol. However, 5,7-DHT treatment produced a 75% depletion of brain serotonin (5-HT) without altering NE concentration and retarded the development of tolerance to ethanol in both measurements. This study with a specific central depletor of 5-HT, without alteration in NE concentration, extends and supports our hypothesis that brain 5-HT modulates the development of tolerance to ethanol.

Key words

Ethanol Tolerance 5,7-Dihydroxytryptamine 5-HT Rat 


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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Anh Dûng Lê
    • 1
  • Jatinder M. Khanna
    • 1
  • Harold Kalant
    • 1
  • A. Eugene LeBlanc
    • 1
  1. 1.Department of PharmacologyUniversity of Toronto and Addiction Research Foundation of OntarioTorontoCanada

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