Psychopharmacology

, Volume 72, Issue 2, pp 205–209 | Cite as

Biochemical and behavioral effects of acute ethanol in rats at different environmental temperatures

  • Larissa A. Pohorecky
  • America E. Rizek
Original Investigations

Abstract

The role of ethanol-induced hypothermia on some selected biochemical and behavioral parameters was evaluated. Rats were kept from 1 h before to 1 h after injection (saline or ethanol 2 g/kg, 20% solution IP) in an environment chamber at either 22°C or 35°C, and then tested behaviorally or sacrificed. Exposure of rats to a warm environment (35°C) prevented the ethanol-induced hypothermia found in rats kept at 22°C. Ethanol-treated rats kept at 35°C had an attenuated increase in levels of free fatty acids and of corticosterone in plasma, suggesting that part of the ethanol-induced response may result from hypothermia rather than from ethanol itself. In addition, tyrosine levels were unexpectedly increased by 18% in intoxicated animals kept at 35°C. By contrast, gross motor activity tested 1 h after injection was depressed more in animals kept at 35°C than in those kept at 22°C, and swim performance was impaired more at 5–20 min after treatment in animals not showing hypothermia. The greater behavioral impairment was not due to any debilitating effects of the warm environment since the saline control animals showed comparable activity to that of rats kept at 22°C. We conclude that ethanol-induced hypothermia may influence and interact with other actions of ethanol.

Key words

Ethanol Behavior Corticosterone NEFA Tyrosine Hypothermia 

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References

  1. Bass MB, Lester D (1978) Swimming as a measure of motor impairment after ethanol and pentobarbital in rats. J Stud Alcohol 39:1618–1622Google Scholar
  2. Beattie D (1978) Physiological changes in rats exposed to cold/restraint stress. Life Sci 23:2307–2314Google Scholar
  3. Chin JH, Goldstein DB (1977) Drug tolerance in biomembranes: A spin label study of the effects of ethanol. Science 196:685–686Google Scholar
  4. Crabbe JC, Rigter H, Uijlen J, Strijbos (1979) Rapid development of tolerance to the hypothermic effect of ethanol in mice. J Pharmacol Exp Ther 208:128–133Google Scholar
  5. Ellis FW (1966) Effect of ethanol on plasma corticosterone levels. J Pharmacol Exp 153:121–128Google Scholar
  6. Ferko AP, Bobyock E (1978) Physical dependence on ethanol: Rate of ethanol clearance from the blood and effect of ethanol on body temperature in rats. Toxicol Appl Pharmacol 46:235–248Google Scholar
  7. Frankel D, Kalant H, Khanna JM, LeBlanc AE (1976) Interaction of propranolol and phentolamine with ethanol in the rat. Can J Physiol Pharmacol 54:622–625Google Scholar
  8. Freund G (1973) Hypothermia after acute ethanol and benzyl alcohol administration. Life Sci 13:345–349Google Scholar
  9. Freund G (1979) Ethanol-induced changes in body temperature and their neurochemical consequences. In: Majchrowicz E, Noble EP (eds) Biochemistry and pharmacology of ethanol, vol 2. Plenum Press, London, pp 439–452Google Scholar
  10. Glick D, Von Redlich D, Levine S (1964) Fluorometric determination of corticosterone and cortisol in 0.02–0.05 milliliters of plasma or submilligram samples of adrenal tissue. Endocrinology 74:653–655Google Scholar
  11. Grieve SJ, Littleton JM (1979) Ambient temperature and the development of functional tolerance to ethanol by mice. J Pharm Pharmacol 31:707–708Google Scholar
  12. Harri M, Tirri R (1969) Brain monoamines in the temperature acclimation of mice. Acta Physiol Scand 75:631–635Google Scholar
  13. Hill MW, Bangham AD (1975) Alcohol, anesthetics and membranes. Adv Exp Med Biol 59:1–9Google Scholar
  14. Hoffman PL, Tabakoff B (1977) Alterations in dopamine receptor sensitivity by chronic ethanol treatment. Nature 268:551–553Google Scholar
  15. Hume DM, Egdahl RH (1959) Effect of hypthermia and of cold exposure on adrenal cortical and medullary secretion. Ann NY Acad Sci 80:435–444Google Scholar
  16. Kahn AU, Forney RB, Hughes FW (1964) Plasma free fatty acids in rats after shock as modified by centrally active drugs. Arch Int Pharmacodyn Ther 151:466–473Google Scholar
  17. Le Mevel JC, Abitbol S, Beraud G, Manley J (1979) Temporal changes in plasma adreno-corticotropin concentration after repeated neurotropic stress in male and female rats. Endocrinology 105:812–817Google Scholar
  18. Li GC, Hahn GM (1978) Ethanol-induced tolerance to heat and to adriamycin. Nature 274:699–701Google Scholar
  19. Lundquist F, Wolthers H (1958) Kinetics of alcohol elimination in man. Acta Pharmacol Toxicol 14:265–289Google Scholar
  20. Pfister HP (1979) The glucocorticosterone response to novelty as a psychological stressor. Physiol Behav 23:649–652Google Scholar
  21. Pohorecky LA, Jaffe LS (1975) Noradrenergic involvement in the acute effects of ethanol. Res Commun Chem Pathol Pharmacol 12:433–448Google Scholar
  22. Pohorecky LA, Newman B, Sun J, Bailey WH (1978) Acute and chronic ethanol ingestion and serotonin metabolism in rat brain. J Pharmacol Exp Ther 199:158–170Google Scholar
  23. Pohorecky LA, Rassi E, Weiss JM, Michalak V (1980) Biochemical evidence for an interaction of ethanol and stress. Preliminary findings. Alc Clin Exp Res 4:423–426Google Scholar
  24. Ritzmann RF, Tabakoff B (1976) Body temperature in mice: A quantitative measure of alcohol tolerance and physical dependence. J Pharmacol Exp Ther 199:158–170Google Scholar
  25. Simmonds MA (1969) Effect of environmental temperature on the turnover of noradrenaline in hypothalamus and other areas of rat brain. J Physiol 203:199–210Google Scholar
  26. Simmonds MA (1970) Effect of environmental temperatures on the turnover of 5-hydroxy-tryptamine in various areas of rat brain. J Physiol 211:93–108Google Scholar
  27. Smith SW (1975) A simple salting out procedure in the colorimetric assay of free fatty acids. Anal Biochem 67:531–539Google Scholar
  28. Stone EA (1970) Behavioral and neurochemical effects of acute swim stress are due to hypothermia. Life Sci 9:877–888Google Scholar
  29. Suzuki M, Tonoue T, Matszuzaki S, Yammamoto K (1967) Initial response of human thyroid, adrenal medulla to acute cold exposure. Can J Physiol Pharmacol 45:423–432Google Scholar
  30. Waalkes TP, Udenfriend (1957) A fluorometric method for the estimation of tyrosine in plasma and tissues. J Lab Clin Med 50:733–736Google Scholar
  31. Weiss B, Laties VG (1961) Behavioral Themoregulation. Science 133:1338–1344Google Scholar
  32. Welch B (1968) Necessity of considering body temperature in drug-cold stress studies of catecholamines. Biochem Pharmacol 17:2013–2015Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Larissa A. Pohorecky
    • 1
    • 2
  • America E. Rizek
    • 1
    • 2
  1. 1.Center of Alcohol StudiesRutgers UniversityNew BrunswickUSA
  2. 2.Department of PharmacologyUniversidad Central de VenezuelaCaracasVenezuela

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