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Urological Research

, Volume 22, Issue 1, pp 33–38 | Cite as

Androgen receptors and hormone sensitivity of a human prostatic cancer cell line (PC-3) are modulated by natural beta-interferon

  • G. Sica
  • G. Dell'Acqua
  • F. Iacopino
  • A. Fattorossi
  • P. Marchetti
  • T. H. van der Kwast
  • M. Pavone-Macaluso
Original Paper

Abstract

Androgen recptors are expressed at a low level in the cell line PC-3, which does not respond to either androgens or antiandrogens. If these cells are exposed to natural beta-interferon (β-IFN) a reduction in cell growth and an increase in androgen receptors, evaluated by both biochemical and immunocytochemical techniques, occur. This increase seems not to be related to a selective block of PC-3 in any phase of the cell cycle. Pretreatment with β-IFN determines in PC-3 cells a partial responsiveness to the androgen dihydrotestosterone as reflected by the increase in cell number. Moreover, the antiandrogen hydroxyflutamide shows agonistic properties by increasing the cell number of PC-3 cells pre-exposed to β-IFN. When the antiandrogen is tested in combination with interferon, it produces a reduction in the β-IFN-induced inhibition of cell growth. It is not known whether these unexpected effects are due to the increase in androgen receptors or to other mechanisms.

Key words

Androgen receptors Hormone sensitivity Hydroxyflutamide Interferon Prostatic cancer 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • G. Sica
    • 1
  • G. Dell'Acqua
    • 1
  • F. Iacopino
    • 1
  • A. Fattorossi
    • 2
  • P. Marchetti
    • 3
  • T. H. van der Kwast
    • 4
  • M. Pavone-Macaluso
    • 5
  1. 1.Istituto di Istologia ed Embriologia Generale, Facoltà di MedicinaUniversità Cattolica del Sacro CuoreRomeItaly
  2. 2.Laboratotio di ImmunologiaDASRSRomeItaly
  3. 3.Dipartimento di Medicina Sperimentale, Oncologia medicaUniversità degli StudiL'AquilaItaly
  4. 4.Department of PathologyErasmus UniversiteitRotterdamThe Netherlands
  5. 5.Istituto di Clinica UrologicaUniversità di PalermoPalermoItaly

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