Advertisement

Virchows Archiv A

, Volume 396, Issue 3, pp 313–317 | Cite as

Peanut lectin: A useful tool for detecting Hodgkin cells in paraffin sections

  • Peter Möller
Original Articles

Summary

Using an extended peroxidase-antiperoxidase method receptors for peanut lectin (PNL) can be visualized in routinely fixed paraffin embedded tissue sections. PNL binding sites are numerous in human tissue. Each tissue, however, displays its specific binding spectrum and cellular binding pattern. 35 cases of Hodgkin's disease containing all histological subtypes were examined. A prominent, constant, and characteristic binding pattern in Hodgkin- and Reed-Sternberg-cells was found. PNL is proposed as an aid for detecting these cells in diagnostic histology. It might turn out to be a very useful reagent particularly in identifying the early lesion in Hodgkin's disease in which Hodgkin cells are small and scarce.

Key words

Peanut lectin Peroxidase antiperoxidase Hodgkin cell 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Graham RC Jr, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302Google Scholar
  2. Janossy G, Duke O, Poulter LW, Panayi G, Bofill M, Goldstein G (1981) Rheumatoid arthritis. A disease of T-lymphocyte/macrophage immunoregulation. Lancet II:839–842Google Scholar
  3. Lotan R, Skutelsky E, Danon D, Sharon N (1975) The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J Biol Chem 250:8518–8523Google Scholar
  4. Müller-Hermelink HK, Schwarting H (1981) Functional studies on histiocytic and interdigitating reticulum cells from human lymphoid tissue. Virchows Arch [Cell Pathol] 37:217–224Google Scholar
  5. Sternberger LA, Hardy PH Jr, Cucitis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry. J Histochem Cytochem 18:315–333Google Scholar
  6. Taylor CR, Burns J (1976) The demonstration of plasma cells and other immunoglobulin containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase labelled antibody. J Clin Pathol 27:14–29Google Scholar
  7. Reisner Y, Gachelin G, Dubois Ph, Nicolas J-F, Sharon N, Jacob F (1977) Interaction of peanut agglutinin, a lectin specific for non-reducing terminal D-galactosyl residues, with embryonal carcinoma cells. Develop Biol 61:20–27Google Scholar
  8. Rose ML, Habeshaw JA, Kennedy R, Sloane J, Willshaw E, Davies AJS (1981) Binding of peanut lectin to germinal-centre cells: a marker for B-cell subsets of follicular lymphoma ? Br J Cancer 44:68–74Google Scholar
  9. Watanabe M, Muramatsu T, Shirane H, Ugai K (1981) Discrete distribution of binding sites for Dolichos biflorus agglutinin (DBA) and for peanut agglutinin (PNA) in mouse organ tissues. J Histochem Cytochem 19:779–790Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Peter Möller
    • 1
  1. 1.Institut für Allgemeine Pathologie und Pathologische Anatomie der Universität HeidelbergHeidelbergFederal Republic of Germany

Personalised recommendations