Advertisement

Mammalian Genome

, Volume 3, Issue 2, pp 112–118 | Cite as

Characterization of a new hybrid mink-mouse clone panel: chromosomal and regional assignments of the GLO, ACY, NP, CKBB, ADH2, and ME1 loci in mink (Mustela vison)

  • Svetlana D. Pack
  • Vladimir M. Bedanov
  • Olga V. Sokolova
  • Natalia S. Zhdanova
  • Natalia M. Matveeva
  • Oleg L. Serov
Original Contributions

Abstract

To expand the mink map, we established a new panel consisting of 23 mink-mouse clones. On the basis of statistical criteria (Wijnen et al. 1977; Burgerhout 1978), we developed a computer program for choice of clones of the panel. Assignments of the following mink genes were achieved with the use of the hybrid panel: glyoxalase (GLO), Chromosome (Chr) 1; acetyl acylase (ACY), Chr 5; creatine phosphokinase B (CKBB), Chr 10; alcohol dehydrogenase-2 (subunit B) (ADH2), Chr 8. Using a series of clones carrying rearrangements involving mink Chr 1 and 8, we assigned the gene for ME1 to the short arm of Chr 1 and that for ADH2 to Chr 8, in the region 8p12-p24. Mapping results confirm the ones we previously obtained with a mink-Chinese hamster panel. However, by means of an improved electrophoretic technique, we revised the localization of the gene for purine nucleoside phosphorylase (NP), which has been thought to be on mink Chr 2. It is reassigned to mink Chr 10.

Keywords

Computer Program Acetyl Creatine Purine Nucleoside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baker, R.F. and Hopkinson, D.A.: Genetic polymorphism of human phosphoglycolate phosphatase (PGP). Ann Hum Genet 42: 143–151, 1978.Google Scholar
  2. Burgerhout, W.G.: A standard procedure for gene assignment by use of somatic cell hybrids. Cytogenet Cell Genet 22: 689–693, 1978.Google Scholar
  3. Chern, C.J., Tan, P., and Park, H.: Chromosomal mapping of human creatine kinase (brain type) using human-rodent somatic cell hybrids. Cytogenet Cell Genet 27: 232–237, 1980.Google Scholar
  4. Gradov, A.A., Pack, S.D., Sukoyan, M.A., Rubtsov, N.B., Bochkarev, M.N., Serov, O.L., Rattazzi, O., Scandalios, J.G., Whitt, G., and Markert, C.L.: Regional assignment of the genes for TK1, GALK, ALDC and ESD on chromosome 8 in the American mink by chromosome-mediated gene transfer. Mol Gen Genet 200: 433–438, 1985.Google Scholar
  5. Holmes, R.S.: Genetics and ontogeny of alcohol dehydrogenase isozymes in the mouse: evidence for a cis-acting regulator gene (Adt-1) controlling C2 isozyme expression in reproductive tissues and close linkage of Adh-3 and Adt-1 on chromosome 3. Biochem Genet 17: 461–472, 1979.Google Scholar
  6. Ikeuchi, T.: Inhibitory effect of ethidium bromide on mitotic chromosome condensation and its application to high-resolution chromosome banding. Cytogenet Cell Genet 38: 56–61, 1984.Google Scholar
  7. Koch, G.A., Eddy, R.L., Haley, L.L., Byers, M.G., McAvoy, M., and Shows, T.B.: Assignment of the human phosphoserine phosphatase gene (PSP) to the pter-q22 region of chromosome 7. Cytogenet Cell Genet 35: 67–69, 1983.Google Scholar
  8. Littlefield, J.W.: Selection of hybrids from matings of fibrblasts in vitro and their presumed recombinants. Science 145: 709–710, 1964.Google Scholar
  9. Mullakandov, M.R., Gradov, A.A., Zakijan, S.M., Rubtsov, N.B., and Serov, O.L.: Peptidases A, B, C, D and S in the American mink: poltmorphism and chromosome localization. Theor Appl Genet 73: 272–277, 1986.Google Scholar
  10. Nesterova, T.B., Borodin, P.M., Zakijan, S.M., and Serov, O.L.: Assignment of the gene for adenine phosphoribosyltransferase on the genetic map of mouse chromosome 8. Biochem Genet 25: 563–568, 1987.Google Scholar
  11. Pack, S.D., Zhdanova, N.S., Sukoyan, M.A., and Serov, O.L.: Chromosomal and regional localization of the genes for UMPH2, APRT, PEPD, PEPS, PSP and PGP in mink: comparison with man and mouse. Cytogenet Cell Genet 50: 127–131, 1989.Google Scholar
  12. Parr, C.W., Bagster, I.A., and Welch, S.G.: Human red cell glyoxalase I polymorphism. Biochem Genet 15: 109–113, 1977.Google Scholar
  13. Povey, S., Inwood, M., Tanyar, A., and Bobrow, M.: The expression of creatine kinase isozymes in human cultured cells. Ann Hum Genet 43: 15–26, 1979.Google Scholar
  14. Rubtsov, N.B., Radjabli, S.I., Gradov, A.A., and Serov, O.L.: Chinese hamster x American mink somatic cell hybrids: characterization of a clone panel and assignment of the mink genes for malate dehydrogenase, NADP-1 and malate dehydrogenase, NAD-1. Theor Appl Genet 60: 99–106, 1981a.Google Scholar
  15. Rubtsov, N.B., Radjabli, S.I., Gradov, A.A., and Serov, O.L.: Chromosome localization of the three syntenic gene pairs in the American mink (Mustela vison). Cytogenet Cell Genet 31: 184–187, 1981b.Google Scholar
  16. Rubtsov, N.B., Gradov, A.A., and Serov, O.L.: Chromosome localization of the loci GOT1, PP, NP, SOD1, PEPA and PEPC in the American mink (Mustela vison). Theor Appl Genet 63: 331–336, 1982.Google Scholar
  17. Serov, O.L. and Pack, S.D.: American mink (Mustela vison). In S.J. O'Brien (ed.), Genetic Maps (Locus Maps of Complex Genomes), pp. 4126–4128, 5th edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1990.Google Scholar
  18. Serov, O.L., Gradov, A.A., Rubtsov, N.B., Zhdanova, N.S., Pack, S.D., Sukoyan, M.A., Mullakandov, M.R., and Zakijan, S.M.: Genetic map of the American mink: gene conservation and organization of chromosomes. In Isozymes: Current Topics in Biological and Medical Research, vol. 15, Genetics, Development, and Evolution, pp. 179–215, Alan R. Liss Inc., New York, 1987.Google Scholar
  19. Sukoyan, M.A., Matveeva, N.M., Belyaev, N.D., Pack, S.S., Gradov, A.A., Shilov, A.G., Zhdanova, N.S., and Serov, O.L.: Cotransfer and phenotypical stabilization of syntenic and asyntenic mink genes into mouse cells by chromosome-mediated gene transfer. Mol Gen Genet 196: 97–104, 1984.Google Scholar
  20. Wijnen, L.M.M., Grzeschik, K.H., Pearson, P.L., and Meera Khan, P.: The human PGM-2 and its chromosomal localization in man-mouse hybrids. Hum Genet 37: 271–278, 1977.Google Scholar
  21. Wilson, D.E., Swallow, D.M., and Povey, S.: Assignment of the human gene for uridine 5′-monophosphate phosphohydrolase (UMPH2) to the long arm of chromosome 17. Ann Hum Genet 50: 223–227, 1986.Google Scholar
  22. Yermolaev, V.I., Karasik, G.I., Khlebodarova, T.M., Matveeva, N.M., Mullakandov, M.R., Nayakshin, A.M., Shumny, T.V., Rubtsov, N.B., Serov, O.L., and Baranov, O.K.: Localization of the α 2-macroglobulin gene and Lpm gene family on mink chromosome 9. Theor Appl Genet 78: 93–96, 1989.Google Scholar

Copyright information

© Springer-Verlag New York Inc 1992

Authors and Affiliations

  • Svetlana D. Pack
    • 1
  • Vladimir M. Bedanov
    • 1
  • Olga V. Sokolova
    • 1
  • Natalia S. Zhdanova
    • 1
  • Natalia M. Matveeva
    • 1
  • Oleg L. Serov
    • 1
  1. 1.Laboratory of Developmental Genetics, Institute of Cytology and GeneticsAcademy of Sciences of the USSR, Siberian BranchNovosibirsk-90Russia

Personalised recommendations