Summary
Previously, we reported a morphological change of Trichophyton violaceum in hair tissue in black dot ringworm. To investigate the morphology of Microsporum canis in human hair tissue, three cases of tinea capitis by M. canis were examined by both light and electron microscopy. The fungal elements, which were located deeply in the keratogenous zone, showed nonseptate hyphae in the outer part of the hair cortex. With the upward development of hair tissues, some hyphae invaded the keratinized inner root sheath and were there transformed into arthrospores, which then occupied the large volume of the inner root sheath; each spore was surrounded by an electron-lucent halo. In some affected hair follicles, at the follicular isthmus level, a microabscess composed of polymorphonuclear leukocytes was often formed in the outer root sheath adjacent to the arthrospores in the keratinized inner root sheath. On the other hand, the remaining hyphae in the cortex became degenerated. Fungi did not invade the hair-germinative cells. There is a distinct relationship between the morphological change of fungi and the differentiation of hair cells in tinea capitis by M. canis as well as in that by T. violaceum, although the direction of invasion and pathological roles of fungal elements within hair tissue are significantly different between the two species of fungi.
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References
Allen HB, Rippon JW (1985) Superficial and deep mycoses. In: Moschella SL, Hurley HJ (eds) Dermatology, vol 1, 2nd edn. Saunders, Philadelphia, pp 739–816
Chandler FW, Kaplan W, Ajello L (1980) A colour atlas and textbook of the histopathology of mycotic diseases. Wolfe Medical, London, p 288
Delektorskii VV, Sheklakov ND, Shadyev KhK (1975) Ultrastructure of M. canis in culture and in affected hair. Vestn Dermatol Venerol 6:10–13
Graham JH, Johnson WC, Burgoon CF, Helwig EB (1964) Tinea capitis, a histopathological and histochemical study. Arch Dermatol 89:528–543
Ikeda M (1964) An electron microscopic study of Trichophyton rubrum. Jpn J Dermatol 74:269–290
Ito M (1986) The innermost cell layer of the outer root sheath in anagen hair follicle: light and electron microscopic study. Arch Dermatol Res 279:112–119
Ito Y, Setoguti T, Nozawa Y, Sakurai S (1967) An electron microscopic observation of Trichophyton violaceum. J Invest Dermatol 48:124–127
Keddie FM (1967) Further investigations of the electron microscopic examination of human hair naturally infected by Microsporum gypseum. Sabouraudia 6:1–4
Kligman AM (1955) Tinea capitis due to M. audouini and M. canis. II. Dynamics of the host-parasite relationship. AMA Arch Dermatol 71:313–337
Nanko H, Watanabe S, Fukuda S (1982) A case report of kerion celsi and ultrastructure of Trichophyton glabrum in vivo and in vitro. Jpn J Dermatol 92:645–656
Okuda C, Ito M, Sato Y (1988) Fungus invasion into human hair tissue in black dot ringworm: light and electron microscopic study. J Invest Dermatol 90:729–733
Orfanos C, Mahrle G, Piroth M (1970) Haarschäden durch Trichophyton schoenleinii im Raster-Elektronenmikroskop vor und nach antimykotischer Behandlung. Klin Wochenschr 48:1111–1119
Pock-Steen B, Kobayasi T (1970) Ultrastructure of the hyphal wall and septum of Trichophyton mentagrophytes. J Invest Dermatol 55:404–409
Puccinelli V, Ceccarelli B (1969) Contribution to the study of the infestation of the hair with tinea mycetes. Arch Klin Exp Dermatol 234:250–260
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212
Rippon JW (1988) Medical mycology the pathogenic fungi and the pathogenic actinomycetes. 3rd edn. Saunders, Philadelphia, pp 186–196
Shelley WB, Shelley ED, Burmeister V (1987) The infected hairs of tinea capitis due to Microsporum canis: demonstration of uniqueness of the hair cuticle by scanning electron microscopy. J Am Acad Dermatol 16:354–361
Takatori K, Udagawa S, Kurata H, Hasegawa A (1983) Microscopic observation of human hairs infected with Microsporum ferrungineum. Mycopathologia 81:129–133
Tosti A, Villardita S, Fazzini ML, Scalici R (1970) Contribution to the knowledge of dermatophytic invasion of hair: an investigation with the scanning electron microscope. J Invest Dermatol 55:123–134
Urabe H, Izu T (1969) The ultrastructure of Trichophyton and a double cell wall in the hypha. J Invest Dermatol 52:508–513
Werner HJ, Jolly HW, Spurlock BO (1966) Electron microscope observations of the fine structure of Microsporum canis. J Invest Dermatol 46:130–134
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Okuda, C., Ito, M., Sato, Y. et al. Fungus invasion of human hair tissue in tinea capitis caused by Microsporum canis: light and electron microscopic study. Arch Dermatol Res 281, 238–246 (1989). https://doi.org/10.1007/BF00431057
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DOI: https://doi.org/10.1007/BF00431057