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An H-2K gene of the t w32mutant at the T/t complex is a close parent of an H-2K qgene

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Abstract

Two recombinant mice have been recovered from the progeny of Ttf/t w32+ animals. They have lost the tw32 lethality factor(s) and gained tufted, presumably from the T chromosome. Southern blot analysis of class I genes of these two new partial t PA027 and t PA286 haplotypes indicates that they have retained at least part of the major histocompatibility complex of the t w32 chromosome (H-2 haplotype H-2 w28). We have prepared a phage library of Eco RI-digested DNA from homozygous t PA027 animals. Upon screening the library with a cDNA probe specific for H-2K genes, we isolated a class I gene displaying all of the distinctive features of a genuine H-2K gene, and which could thus be defined as an H-2K w28 gene. The H-2K w28 gene is 92–95% homologous to H-2K band H-2K dgenes and differs significantly from the other class I genes sequenced so far. Homology with the H-2K bsequence reaches nearly 100% in the 3′ part of the H-2K w28 gene. Moreover, the homology with an H-2K qcDNA sequence reaches 99.8%. Several hypotheses can account for the near identity of H-2K b, H-2K q,and H-2K w28 gene sequences: either recombination between H-2 w28 and H-2 band H-2 qsequences occurred before or at the.time the strain was established, or the class I genes of the t w32 chromosome and the H-2 band H-2 qgenes found in inbred strains of mice have separated from each other rather recently.

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  1. Takashi Morita

    Present address: Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-Oka, Suita Osaka, Japan

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  1. Unité de Biologie Moléculaire du Gène, U. 277 I.N.S.E.R.M. and E.R. C.N.R.S. No. 201, Institut Pasteur, 25, rue du Dr. Roux, 75724, Paris Cédex 15, France

    Takashi Morita, Christiane Delarbre, Philippe Kourilsky & Gabriel Gachelin

  2. Laboratoire d'Oncologie Moléculaire, I.R.S.C., B.P. No. 8, 94802, Villejuif Cédex, France

    Michel Kress

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Morita, T., Delarbre, C., Kress, M. et al. An H-2K gene of the t w32mutant at the T/t complex is a close parent of an H-2K qgene. Immunogenetics 21, 367–383 (1985). https://doi.org/10.1007/BF00430802

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