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H-2 control of expression of an idiotype shared by normal B cells and a B-cell lymphoma

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Abstract

The spleens of normal B10,H-2 a H-44b p/Wts (2a4b) mice; contain cells which, in response to mitogen stimulation, secrete hemolytic antibody specific for a determinant present on both sheep and bromelain-treated mouse erythrocytes. These cells were found to be Ly-1 positive. Approximately 50% of these cells bear surface immunoglobulin (sIg) with the same idiotype as the sIg of a 2a4b-derived B-cell lymphoma, CH12. Backcross analysis revealed H-2 control of the frequency of the idiotype-positive B cell. The regulatory gene did not correlate with the Igh-1 allotype, and analysis of 22 inbred mouse strains mapped the gene to the I-E subregion. Surprisingly, only strains homozygous for E kα expressed the idiotype, and expression was a recessive trait. Possible mechanisms for this control of idiotype expression and its relation to lymphomagenesis are discussed.

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Abbreviations

2 a4b :

B10.H-2 aH-4bp/Wts

Br-MRBC:

bromelain-treated mouse erythrocytes

C:

complement

LPS:

lipopolysaccharide W

pfc:

plaque-forming cells

sIg:

surface immunoglobulin

SRBC:

sheep erythrocytes

Ts:

T suppressor.

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Authors and Affiliations

  1. Department of Microbiology and Immunology and the Lineberger Cancer Research Center, The University of North Carolina, 27514, Chapel Hill, North Carolina

    Gail Abendroth Bishop & Geoffrey Haughton

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  1. Gail Abendroth Bishop
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  2. Geoffrey Haughton
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Bishop, G.A., Haughton, G. H-2 control of expression of an idiotype shared by normal B cells and a B-cell lymphoma. Immunogenetics 21, 355–366 (1985). https://doi.org/10.1007/BF00430801

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