Fresenius' Zeitschrift für analytische Chemie

, Volume 269, Issue 5, pp 363–367 | Cite as

Optical rotatory dispersion in quantitative analysis: Microdetermination of some steroid sex-hormones

  • Saad S. M. Hassan
Original Papers


A method is described for microdetermination of some steroid sex-hormones based on the optical properties of these compounds. The optical rotatory dispersion spectra of testosterone, progesterone, estrone and androstanalone show rotation maxima at 250, 300, 310 and 305 nm, respectively. The rotation values are proportional, in linear relations, to the hormone concentration ranging from 15 μg up to 2.5 mg/ml. Binary mixtures of some of these hormones are also simultaneously determined by rotation measurement at two wave lengths. The results show an average recovery of 99.8%, the maximum absolute error being ± 2%.

Optische Rotationsdispersion in der quantitativen Analyse: Mikrobestimmung einiger Steroid-Sexualhormone


Die Rotationsdispersionsspektren von Testosteron, Progesteron, Oestron und Androstanalon weisen Rotations-maxima bei 250, 300, 310 und 305 nm auf. Die Rotationswerte sind der Hormonkonzentration von 15 μg bis 2,5 mg/ml linear proportional. Binäre Gemische dieser Hormone können durch Rotationsmessung bei zwei Wellenlängen simultan bestimmt werden. Durchschnittlich werden 99,8% wiedergefunden, der maximale Fehler beträgt ± 2% absolut.

Best. von Hormonen, Testosteron, Progesteron, Oestron, Androstanalon Rotationsdispersion Steroid-Sexual-hormone 


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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Saad S. M. Hassan
    • 1
  1. 1.Research Microanalytical Laboratory, Department of Chemistry, Faculty of ScienceAin Shams UniversityCairoEgypt

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