, Volume 22, Issue 1, pp 35–48 | Cite as

A structural somatic variant of the Kk antigen is generated by point mutation

  • Günther Karmann
  • Konrad T. Beyreuther
  • Matthias Cramer
  • Bodo Holtkamp
  • Susanne Proksa
  • Klaus Rajewsky


We have previously selected structural variants of the Kk antigen from a (C3 × D2)F1 T-cell lymphoma. Those mutants were identified by the loss of certain epitopes defined by monoclonal antibodies. The variant Kk molecule from HK13.S3 cells is no longer recognized by 40% of the trinitrophenyl-specific, Kk-restricted cytotoxic T lymphocytes. Here we report on the primary structure of the altered Kk molecules from the cell line HK13.S3. Comparison with the parental Kk reveals a single base pair exchange, GCG to GTG, that results in an alanine to valine exchange in position 40 of the protein. This observation emphasizes that minor structural alterations in class I molecules may have a strong effect on the H-2-restricted T-cell response.


Lymphoma Monoclonal Antibody Alanine Base Pair Point Mutation 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Günther Karmann
    • 1
  • Konrad T. Beyreuther
    • 1
  • Matthias Cramer
    • 1
  • Bodo Holtkamp
    • 1
  • Susanne Proksa
    • 1
  • Klaus Rajewsky
    • 1
  1. 1.Institute for GeneticsCologne 41Germany

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