Virchows Archiv A

, Volume 392, Issue 1, pp 21–31 | Cite as

Substance P in an argentaffin carcinoid of the caecum: Biochemical and biological characterization

  • M. Ratzenhofer
  • R. Gamse
  • H. Höfler
  • L. Auböck
  • H. Popper
  • P. Pohl
  • F. Lembeck
Original Articles

Summary

An argentaffin carcinoid tumour of the caecum which contained serotonin (167 μg/g) and consisted predominantly of EC1-cells, was analysed for the presence of peptides using immunohistochemical, biochemical and pharmacological methods.

A very high content of 3.9 μg/g of immunoreactive substance P was found. The distribution of cells staining positively for substance P matched that of cells containing serotonin. While some immunoreactive somatostatin (3.2 ng/g) was present in the tumour, neurotensin, glucagon, gastrin, and motilin were not found.

Part of the substance P immunoreactivity measured most likely represents authentic substance P: it behaved like substance P in two Chromatographic systems and in two bioassays, and its activity on the guinea pig ileum was abolished by specific tachyphylaxis towards substance P.

Key words

Carcinoid tumour substance P 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • M. Ratzenhofer
    • 1
  • R. Gamse
    • 2
  • H. Höfler
    • 1
  • L. Auböck
    • 1
  • H. Popper
    • 1
  • P. Pohl
    • 3
  • F. Lembeck
    • 2
  1. 1.Dept. of PathologyGrazAustria
  2. 2.Department of Experimental and Clinical PharmacologyUniversity of GrazAustria
  3. 3.Department of SurgeryBarmherzige Brüder HospitalGrazAustria

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