Summary
A pattern of active accumulation of trehalose during growth on glucose medium, TAC(+) phenotype, is controlled by a polymeric series of maltose fermentation (MAL) genes. An essential requirement for expression of the TAC(+) phenotype is that the MAL gene be in the constitutive state, MAL c. Mutation of a constitutive MAL allele to a maltose- inducible or nonfermenting (mal) state, alters the pattern of trehalose metabolism so that little or no trehalose accumulation occurs during growth on glucose medium. The TAC(+) phenotype is obtained in MAL c strains whether or not α-glucosidase formation is sensitive or resistant to carbon catabolite repression. However, trehalose accumulation is sensitive to glucose levels even in MAL c strains in which α-glucosidase formation is insensitive to catabolite repression. The effects of constitutive MAL genes on trehalose accumulation cannot be accounted for by an increase in trehalose-6 phosphate synthase or a decrease in trehalase as determined in vitro. A mechanism is proposed in which the gene-product of a MAL gene serves as a common positive regulator for expression of four genes coding respectively for maltose permease, maltase, α-methylglucosidase and a component of the trehalose accumulation system.
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Paper I appeared in Cell. and Molec. Biology 25: 345–354, 1979
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de Oliveira, D.E., Rodrigues, E.G.C., Mattoon, J.R. et al. Relationships between trehalose metabolism and maltose utilization in Saccharomyces cerevisiae . Curr Genet 3, 235–242 (1981). https://doi.org/10.1007/BF00429826
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DOI: https://doi.org/10.1007/BF00429826