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Intraspecific variation in the structural organization and redundancy of chloroplast ribosomal DNA cistrons in Euglena gracilis

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Summary

Chloroplast DNAs from six different laboratory collections of Euglena gracilis “strain Z” and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosomal DNA cistron number (one or three) and structural organization among those strains designated as “strain Z” and bacillaris. One culture previously designated as “Z” is most likely bacillaris.

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References

  • Avadhani NG, Buetow DE (1972) Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis. Biochem J 128:353–365

    Google Scholar 

  • Bedbrook JR, Bogorad L (1976) Endonuclease recognition sites mapped on Zea mays chloroplast DNA. Proc Natl Acad Sci USA 73:4309–4313

    Google Scholar 

  • Bedbrook JR, Kolodner R (1979) The structure of chloroplast DNA. Ann Rev Plant Physiol 30:593–620

    Google Scholar 

  • Bedbrook JR, Kolodner R, Bogorad L (1977) Zea mays chloroplast ribosomal RNA genes are part of a 22,000 base pair inverted repeat. Cell 11:739–749

    Google Scholar 

  • Buetow DE (1965) Growth, survival and biochemical alteration of Euglena gracilis in medium limited in sulfur. J Cell Comp Physiol 66:235–242

    Google Scholar 

  • Buetow DE (1981) In: Govindjee (ed) Photosynthesis, vol II. Academic Press, New York (in press)

    Google Scholar 

  • Cullis CA, Davies DR (1975) Ribosomal DNA amounts in Pisum sativum. Genetics 81:485–492

    Google Scholar 

  • Flavell RB, Smith DB (1974) Variation in nucleolar organizer rRNA gene multiplicity in wheat and rye. Chromosoma 47:327–334

    Google Scholar 

  • Gilham NW (1978) Organelle heredity. Raven Press, New York, p 602

    Google Scholar 

  • Givens JF, Phillips RL (1976) The nucleolus organizer region of maize (Zea mays L,), Ribosomal RNA gene distribution and nucleolar interactions. Chromosoma 57:103–117

    Google Scholar 

  • Gray PW, Hallick RB (1977) Restriction endonuclease map of Euglena gracilis chloroplast DNA. Biochemistry 16:1665–1669

    Google Scholar 

  • Gray PW, Hallick RB (1978) Physical mapping of the Euglena gracilis chloroplast DNA and ribosomal RNA gene region. Biochemistry 17:284–289

    Google Scholar 

  • Gray PW, Hallick RB (1979) Isolation of Euglena gracilis chloroplast 5S ribosomal RNA and mapping the 5S rRNA gene on chloroplast DNA. Biochemistry 18:1820–1825

    Google Scholar 

  • Helling RB, El-Gewely MR, Lomax MI, Baumgartner JE, Schwartzbach SD, BArnett WE (1979) Organization of the chloroplast ribosomal RNA genes of Euglena gracilis bacillaris. Mol Gen Genet 174:1–10

    Google Scholar 

  • Herrmann RG, Bohnert HJ, Driesel A, Hobom G (1976) In: Bücher Th, Neupert W, Sebald W, Werner S (eds) Genetics and biogenesis of chloroplasts and mitochondria. North Holland, Amsterdam, pp 351–359

    Google Scholar 

  • Hobom G, Bohnert HJ, Driesel A, Herrmann RG (1977) In: Bogorad L, Weil JH (eds) Acides nucléiques et synthése des protéines chez les végétaux. C.N.R.S., Paris, pp 63–69

    Google Scholar 

  • Jenni B, Stutz E (1978) Physical mapping of the ribosomal DNA region of Euglena gracilis chloroplast DNA. Eur J Biochem 88:127–134

    Google Scholar 

  • Jenni B, Stutz E (1979) Analysis of Euglena gracilis chloroplast DNA Mapping of a DNA sequence complementary to 16S rRNA outside of the three rRNA gene sets. FEBS Lett 102:95–99

    Google Scholar 

  • Kissil MS, Buctow DE (1974) Mass isolation of chloroplasts for preparation of polyribosomes. J Cell Biol 63:169a

    Google Scholar 

  • Lomax MI, Helling RB, Hecker LI, Schwartzbach SD, Barnett WE (1977) Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis. Science 196:202–205

    Google Scholar 

  • Maggini F, Blanco A, Licci D, Carmona MJ (1980) Amount of ribosomal DNA and seed protein content in the genus Triticum. Experientia 36:1047–1048

    Google Scholar 

  • McCrea JM, Hershberger CL (1976) Chloroplast DNA codes for transfer RNA. Nucleic Acids Res 3:2005–2018

    Google Scholar 

  • Mego JL (1974) The effect of hadicidin on chloroplast development in non-dividing Euglena cells. Biochem Biophys Acta 79:221–225

    Google Scholar 

  • Mielenz JR, Milner JJ, Herschberger CL (1977) Analysis of Euglena gracilis chloroplast deoxyribonucleic acid with a restriction endonuclease. J Bacteriol 130:860–868

    Google Scholar 

  • Mohan J, Flavell RB (1974) Ribosomal RNA cistron multiplicity and nucleolar organizers in hexaploid wheat. Genetics 76:33–44

    Google Scholar 

  • Ramirez SA, Sinclair JH (1975) Intraspecific variation of ribosomal gene redundancy in Zea mays. Genetics 80:495–504

    Google Scholar 

  • Rawson JRY, Boerma CL (1979) Hybridization of EcoRI chloroplast DNA fragments to pulse labeled RNA from different stages of chloroplast development. Biochem Biophys Res Comm 89:743–749

    Google Scholar 

  • Rawson JR, Haselkorn R (1973) Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis. J Mol Biol 77:125–132

    Google Scholar 

  • Rawson JRY, Kushner SR, Vapnek D, Alton MK, Boerma CL (1978) Chloroplast ribosomal genes in Euglena gracilis exist as three clustered tandem repeats. Gene 3:191–209

    Google Scholar 

  • Rochaix JD (1978) Restriction endonuclease map of the chloroplast DNA of Chlamydomonas reinhardii. J Mol Biol 126:597–617

    Google Scholar 

  • Sager R, Schlanger G (1976) In: King RC, (ed) Handbook of genetics, vol. 5, Molecular genetics. Plenum Press, New York, pp 371–423

    Google Scholar 

  • Scott NS (1973) Ribosomal RNA cistrons in Euglena gracilis. J Mol Biol 81:327–336

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Starr RC (1978) The culture collection of algae at the University of Texas at Austin. J Phycol 14 Suppl:47–100

    Google Scholar 

  • Stutz E, Vandrey JP (1971) Ribosomal DNA satellite of Euglena gracilis chloroplast DNA. FEBS Lett 17:277–280

    Google Scholar 

  • Stutz E, Crouse EJ, Graf L, Jenni B, Kopecka H (1976) In: Bücher Th, Neupert W, Sebald W, Werner S (eds) Genetics and biogenesis of chloroplasts and mitochondria. North-Holland, Amsterdam, pp 339–345

    Google Scholar 

  • Swanstrom R, Shank PR (1978) X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I. Anal Biochem 86:184–192

    Google Scholar 

  • Vacek AT, Bourque DP (1980) Mode of inheritance and evidence for cistron heterogenity of chloroplast 16S ribosomal RNA genes in Nicotiana. Plasmid 4:205–214

    Google Scholar 

  • Yampol JH, Pospelov VA (1978) Content of rDNA in the clones of onion Allium frstulosum with various morphology of satellite nucleolar chromosomes. Genetika 14:406–415

    Google Scholar 

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Author notes

  1. Edwin A. Wurtz

    Present address: Laboratory of Radiation Ecology, Fisheries Center, WH-10, University of Washington, 98105, Seattle, Washington, USA

Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Illinois, 61801, Urbana, Illinois, USA

    Edwin A. Wurtz & Dennis E. Buetow

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  1. Edwin A. Wurtz
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  2. Dennis E. Buetow
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Wurtz, E.A., Buetow, D.E. Intraspecific variation in the structural organization and redundancy of chloroplast ribosomal DNA cistrons in Euglena gracilis . Curr Genet 3, 181–187 (1981). https://doi.org/10.1007/BF00429820

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  • DOI: https://doi.org/10.1007/BF00429820

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