, Volume 160, Issue 5, pp 464–468 | Cite as

Internode length in Zea mays L.

The dwarf-1 mutation controls the 3β-hydroxylation of gibberellin A20 to gibberellin A1
  • Clive Spray
  • Bernard O. Phinney
  • Paul Gaskin
  • Sarah J. Gilmour
  • Jake MacMillan


[13C, 3H]Gibberellin A20 (GA20) has been fed to seedlings of normal (tall) and dwarf-5 and dwarf-1 mutants of maize (Zea mays L.). The metabolites from these feeds were identified by combined gas chromatography-mass spectrometry. [13C, 3H]Gibberellin A20 was metabolized to [13C, 3H]GA29-catabolite and [13C, 3H]GA1 by the normal, and to [13C, 3H]GA29 and [13C, 3H]GA1 by the dwarf-5 mutant. In the dwarf-1 mutant, [13C, 3H]GA20 was metabolized to [13C, 3H]GA29 and [13C, 3H]GA29-catabolite; no evidence was found for the metabolism of [13C, 3H]GA20 to [13C, 3H]GA1. [13C, 3H]Gibberellin A8 was not found in any of the feeds. In all feeds no dilution of 13C in recovered [13C, 3H]GA20 was observed. Also in the dwarf-5 mutant, the [13C]label in the metabolites was apparently undiluted by endogenous [13C]GAs. However, dilution of the [13C]label in metabolites from [13C, 3H]GA20 was observed in normal and dwarf-1 seedlings. The results from the feeding studies provide evidence that the dwarf-1 mutation of maize blocks the conversion of GA20 to GA1.

Key words

Gibberellin metabolism Mutant (dwarf, ZeaStem elongation Zea (gibberellin metabolism) 



gibberellin An


combined gas chromatography-mass spectrometry


high-performance liquid chromatography


reverse phase


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Clive Spray
    • 1
  • Bernard O. Phinney
    • 1
  • Paul Gaskin
    • 2
  • Sarah J. Gilmour
    • 2
  • Jake MacMillan
    • 2
  1. 1.Department of BiologyUniversity of CaliforniaLos Angeles
  2. 2.A.R.C. Research Group, School of ChemistryUniversity of BristolBristolUK

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