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Isolation of biochemical mutants using haploid mesophyll protosplasts of Hyoscyamus muticus

IV. Biochemical characterisation of nitrate non-utilizing clones

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Abstract

Four nitrate non-utilizing clones of Hyoscyamus muticus, obtained by a total isolation method, possess all the known characteristics of cnx-type nitrate-reductase-deficient variants: 1) strict dependence on a reduced nitrogen source such as a mixture of amino acids; 2) chlorate resistance; 3) normal nitrate uptake; 4) lack of nitrate-reductase and xanthine-dehydrogenase activities, but presence of cytochrome-c-reductase and nitrite-reductase activities; 5) in vitro nitrate-reductase complementation with a molybdenum cofactor source. Two of the clones (MA-2 and I2D12) are molybdate repairable in vivo, whilst the other two clones (VIC2 and XIVE9) are not.

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Abbreviations

CCR:

cytochrome-c reductase

Mo-Co:

molybdenum cofactor

MVH:

reduced methylviologen

NiR:

nitrite reductase

NR:

nitrate reductase

XDH:

xanthine dehydrogenase

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Authors and Affiliations

  1. Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland

    Heinz Fankhauser, Franziska Bucher & Patrick J. King

Authors
  1. Heinz Fankhauser
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  2. Franziska Bucher
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  3. Patrick J. King
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Fankhauser, H., Bucher, F. & King, P.J. Isolation of biochemical mutants using haploid mesophyll protosplasts of Hyoscyamus muticus . Planta 160, 415–421 (1984). https://doi.org/10.1007/BF00429757

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  • DOI: https://doi.org/10.1007/BF00429757

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