Summary
The effect of the administration of anti-insulin serum to newborn rats, surgically delivered under ether anaesthesia at term, was examined with respect to liver glycogen concentration and plasma concentrations of glucose, lactate and free fatty acids. Newborn rats thus treated showed decreased liver glycogen concentrations and elevated plasma concentrations of glucose, lactate and free fatty acids compared to untreated control animals one hour later. These effects were dose-dependent with respect to the amount of anti-insulin serum administered. The simultaneous administration of glucagon with anti-insulin serum at birth was no more effective in mobilising glycogen stores than anti-insulin serum alone, although plasma glucose concentrations in these animals were higher and plasma lactate concentrations were lower. Either anti-insulin serum or glucagon abolished the postnatal hypoglycaemia observed in untreated neonatal rats. The rate of fall in plasma lactate concentrations after birth was stimulated in glucagon-treated rats but was retarded in rats treated with anti-insulin serum. Hormonal control over the initiation of glycogenolysis and gluconeogenesis in the newborn rat appears to be different, a fall in plasma insulin being the prime factor involved in triggering glycogen mobilization and a rise in plasma glucagon the prime event that initiates gluconeogenesis.
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Dawkins, M.J.R.: Glycogen synthesis and breakdown in foetal and newborn rat liver. Ann. N. Y. Acad. 111, 203–211 (1963)
Yeung, D., Oliver, I.T.: Factors affecting the premature induction of phosphopyruvate carboxylase in neonatal rat liver. Biochem. J. 108, 325–331 (1968)
Cake, M.H., Yeung, D., Oliver, I.T.: The control of postnatal hypoglycaemia. Biol. Neonate 18, 183–192 (1971)
Snell, K., Walker, D.G.: Glucose metabolism in the newborn rat: temporal studies in vivo. Biochem. J. 132, 739–752 (1973)
Ballard, F.J.: Gluconeogenesis and the regulation of blood glucose in the neonate. In: R.R. Rodriguez, J. Vallance-Owen (Eds.): Diabetes, pp. 592–600. Amsterdam: Excerpta Medica Foundation (1971)
Girard, J.R., Cuendet, G.S., Marliss, E.B., Kervran, A., Rieutort, M., Assan, R.: Fuels, hormones, and liver metabolism at term and during the early postnatal period in the rat. J. Clin. Invest. 52, 3190–3200 (1973)
Ballard, F.J.: The development of gluconeogenesis in rat liver. Controlling factors in the newborn. Biochem. J. 124, 265–274 (1971)
Snell, K., Walker, D.G.: The adaptive behaviour of isoenzyme forms of rat liver alanine aminotransferases during development. Biochem. J. 128, 403–413 (1972)
Cowgill, R.W., Pardee, A.B.: Isolation and hydrolysis of liver glycogen. In: Experiments in biochemical research techniques, pp. 158–159. New York: Wiley & Sons 1957
Vernon, R.G., Walker, D.G.: Glycerol metabolism in the neonatal rat. Biochem. J. 118, 531–536 (1970)
Krebs, H.A., Bennett, D.A.H., de Gasquet, P., Gascoyne, T., Yoshida, T.: Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat kidney. Biochem. J. 86, 22–27 (1963)
Hohorst, H.J.: L-lactate. In: H.U. Bergmeyer (Ed.): Methods of Enzymatic Analysis, pp. 266–270. New York: Academic Press 1963
Chernick, S., Novak, M.: Effect of insulin on FFA mobilization and ketosis in fasting pregnant rats. Diabetes 19, 563–570 (1970)
Vernon, R.G., Walker, D.G.: Glucose metabolism in the developing rat, studies in vivo. Biochem. J. 127, 521–529 (1972)
Portha, B., Rosselin, G., Picon, L.: Postmaturity in the rat: impairment of insulin, glucagon, and glycogen stores. Diabetologia 12, 429–436 (1976)
Gain, K., Watts, C.: Blood glucose, plasma insulin and hepatic glycogen in the newborn rat. Biol. Neonate 30, 35–39 (1976)
Kervran, A., Gilbert, M., Girard, J.R., Assan, R., Jost, A.: Effect of environmental temperature on glucose-induced insulin response in the newborn rat. Diabetes 25, 1026–1030 (1976)
Snell, K., Walker, D.G.: Glucose metabolism in the newborn rat: hormonal effects in vivo. Biochem. J. 134, 899–906 (1973)
Mackrell, D.J., Sokal, J.E.: Antagonism between the effects of insulin and glucagon on the isolated liver. Diabetes 18, 724–732 (1969)
Glinsman, W.H., Mortimore, G.E.: Influence of glucagon and 3′, 5′-AMP on insulin responsiveness of the perfused rat liver. Am. J. Physiol. 215, 553–559 (1968)
Exton, J.H., Lewis, S.B., Ho, R.J., Robison, G.A., Park, C.R.: The role of cyclic AMP in the interaction of glucagon and insulin in the control of liver metabolism. Ann. N. Y. Acad. Sci. 185, 85–100 (1971)
Stalmans, W., de Wulf, H., Hue, L., Hers, H.-G.: The sequential inactivation of glycogen phosphorylase and activation of glycogen synthetase in liver after the administration of glucose to mice and rats. Eur. J. Biochem. 41, 127–134 (1974)
Pines, M., Bashan, N., Moses, S.W.: Glucose effect on glycogen synthetase and phosphorylase in fetal rat liver. FEBS Lett. 62, 301–303 (1976)
Pines, M., Bashan, N., Moses, S.W.: Effect of hydrocortisone and glucagon on glycogen metabolism in the fetal rat liver. Biochim. Biophys. Acta 411, 369–376 (1975)
Devos, P., Hers, H.-G.: Glycogen metabolism in the liver of the foetal rat. Biochem. J. 140, 331–340 (1974)
Hunter, D.J.S.: Changes in blood glucose and liver carbohydrate after intrauterine injection of glucagon into foetal rats. J. Endocrinol. 45, 367–374 (1969)
Greengard, D., Dewey, H.K.: The premature deposition or lysis of glycogen in livers of fetal rats injected with hydrocortisone or glucagon. Dev. Biol. 21, 452–461 (1970)
Girard, J.R., Caquet, D., Bal, D., Guillet, I.: Control of rat liver enzymes by insulin and glucagon during the perinatal period. Enzyme 15, 272–285 (1973)
Chiu, H.F., Phillips, M.J.: Glucagon induces premature structural differentiation of fetal hepatocytes. Lab. Invest. 30, 305–310 (1974)
Kotoulas, O.B., Phillips, M.J.: Fine structural aspects of the mobilisation of hepatic glycogen, I. Acceleration of glycogen breakdown. Am. J. Pathol. 63, 1–21 (1971)
Kotoulas, O.B., Ho, J., Adachi, F., Weigensberg, B.I., Phillips, M.J.: Fine structural aspects of the mobilisation of hepatic glycogen, II. Inhibition of glycogen breakdown. Am. J. Pathol. 63, 23–26 (1971)
Van de Werve, G., Hue, L., Hers, H.-G.: Hormonal and ionic control of the glycogenolytic cascade in rat liver. Biochem. J. 162, 135–142 (1977)
Van de Werve, G., Stalmans, W., Hers, H.-G.: The effect of insulin on the glycogenolytic cascade and on the activity of glycogen synthetase in the liver of anaesthetised rabbits. Biochem. J. 162, 143–146 (1977)
Girard, J.R., Kervran, A., Assan, R.: Functional maturation of the A-cell in the rat. In: R.A. Camerine-Da Valos, H.S. Cole (Eds.): Early Diabetes in Early Life, pp. 57–71. New York: Academic Press 1975
Philippidis, H., Ballard, F.J.: The development of gluconeogenesis in rat liver. Effects of glucagon and ether. Biochem. J. 120, 385–392 (1970)
Hahn, P., Novak, M.: Development of brown and white adipose tissue. J. Lipid Res. 16, 79–91 (1975)
Hardman, M.J., Hull, D.: The action of insulin on brown adipose tissue in vivo. J. Physiol. 221, 85–92 (1972)
Hardman, M.J., Hull, D., Milner, R.D.: Brown adipose tissue metabolism in vivo and serum insulin concentrations in rabbits soon after birth. J. Physiol. 213, 175–183 (1971)
Mulligan, P.B., Schwartz, R.: Hepatic metabolism in neonatal hypoglycaemia. Am. J. Dis. Child. 102, 490–496 (1961)
Pribylora, H., Rylander, E.: Free fatty acids, glycerol, glucose and β-hydroxybutyrate of plasma of infants protected from cooling and exposed to cold at various times after birth. Biol. Neonate 20, 425–435 (1972)
Sperling, M.A., DeLamater, P.V., Phelps, D., Fiser, R.H., Oh, W., Fisher, D.A.: Spontaneous and amino acid-stimulated glucagon secretion in the immediate postnatal period. J. Clin. Invest. 53, 1159–1166 (1974)
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Snell, K., Walker, D.G. Glucose metabolism in the newborn rat: The role of insulin. Diabetologia 14, 59–64 (1978). https://doi.org/10.1007/BF00429709
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DOI: https://doi.org/10.1007/BF00429709