Summary
Four subjects were studied during exercise at 50% of maximum oxygen uptake after a normal diet, after a low carbohydrate (CHO) diet following exercise-induced glycogen depletion, and after a high CHO diet. This regime has previously been shown to cause changes in the amount of glycogen stored in the exercising muscles. Metabolic and respiratory parameters were measured during the exercise. The respiratory exchange ratio, blood lactate, blood pyruvate, blood glucose and plasma triglycerides were lower than normal following the low CHO diet and higher than normal following the high CHO diet. Plasma free fatty acids and plasma glycerol were higher than normal after the low CHO diet and lower than normal after the high CHO diet. The contribution of CHO to metabolism was less than normal after the low CHO diet and greater than normal after the high CHO diet. The altered availability of FFA does not appear to be a result of the variations in the blood lactate content.
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Armstrong, D. T., Steele, R., Altszuler, N., Dunn, A., Bishop, J. S., de Bodo, R. C.: Regulation of plasma free fatty acid turnover. Amer. J. Physiol. 201, 9–15 (1961)
Berger, M., Hagg, S. A., Goodman, M. N., Ruderman, N. B.: Glucose metabolism in perfused skeletal muscle. Effects of starvation, diabetes, fatty acids, acetoacetate insulin and exercise on glucose uptake and disposition. Biochem. J. 158, 191–202 (1976)
Bergstrom, J., Hultman, E.: Muscle glycogen synthesis after exercise: an enhancing factor localised to the muscle cells in man. Nature (Lond.) 210, 309–310 (1966)
Bergstrom, J., Hermansen, L., Hultman, E., Saltin, B.: Diet, muscle glycogen and physical performance. Acta physiol. scand. 71, 140–150 (1967)
Bjorntorp, P.: The effect of lactic acid on adipose tissue metabolism in vitro. Acta med. scand. 178, 253–255 (1965)
Boyd, A. E., Giamber, S. R., Mager, M., Lebovitz, H. E.: Lactate inhibition of lipolysis in exercising man. Metabolism 23, 531–542 (1974)
Dole, V. P., Meinertz, H.: Microdetermination of long-chain fatty acids in plasma and tissues. J. biol. Chem. 235, 2595–2599 (1960)
Fredrikson, D. S., Gordon, R. S.: Transport of fatty acids. Physiol. Rev. 38, 585–630 (1958)
Gollnick, P. D., Piehl, K., Saubert, C. W., Armstrong, R. B., Saltin, B.: Diet, exercise and glycogen changes in human muscle fibres. J. appl. Physiol. 33, 421–425 (1972)
Goodman, M. N., Berger, M., Ruderman, N. B.: Glucose metabolism in rat skeletal muscle at rest. Effect of starvation, diabetes, ketone bodies and free fatty acids. Diabetes 23, 881–888 (1974)
Hultman, E.: Studies on muscle metabolism of glycogen and active phosphate in man with special reference to exercise and diet. Scand. J. clin. Lab. Invest. 19, (Suppl.) 94, 1–63 (1967)
Issekutz, B., Miller, H. I., Paul, P., Rodahl, K.: Effect of lactic acid on free fatty acids and glucose oxidation in dogs. Am. J. Physiol. 209, 1137–1144 (1965)
Issekutz, B., Shaw, W. A., Issekutz, T. B.: Effect of lactate on FFA and glycerol turnover in resting and exercising dogs. J. appl. Physiol. 39, 349–353 (1975)
Kelman, G. R., Maughan, R. J., Williams, C.: The effect of dietary modifications on blood lactate during exercise. J. Physiol. (Lond.) 251, 34P-35P (1975)
Laurell, S., Tibbling, G.: An enzymatic fluorimetric micro-method for the determination of glycerol. Clin. chim. Acta 13, 317–322 (1966)
McCance, R. A., Widdowson, E. D.: The composition of foods. MRC Special Report Series No. 297. London: H.M.S.O. 1960
Newsholme, E. A., Randle, P. J., Manchester, K. L.: Inhibition of the phosphofructokinase reaction in perfused rat heart by respiration of ketone bodies, fatty acids and pyruvate. Nature (Lond.) 193, 270–271 (1962)
Olsen, C.: An enzymatic fluorimetric micromethod for the determination of acetoacetate, Β-Hydroxybutyrate, pyruvate and lactate. Clin. chim. Acta 33, 293–300 (1971)
Paul, P.: Effects of long-lasting physical exercise and training on lipid metabolism. In: Metabolic adaptation to prolonged physical exercise: Proceedings of the Second International Symposium on Biochemistry of Exercise, Magglingen 1973 (H. Howald, J. R. Poortmans, eds.), pp. 156–193. Basel: BirkhÄuser 1975
Ramadoss, C. S., Uyeda, K., Johnston, J. M.: Studies on the fatty acid inactivation of phosphofructokinase. J. biol. Chem. 251, 98–107 (1976)
Randle, P. J., Garland, P. B., Hales, C. N., Newsholme, E. A.: The glucose-fatty acid cycle. Its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet I, 785–789 (1963)
Randle, P. J., Newsholme, E. A., Garland, P. B.: Regulation of glucose uptake by muscle, 8. Effects of fatty acids, ketone bodies and pyruvate, and of alloxan diabetes and starvation, on the uptake and metabolic fate of glucose in rat heart and diaphragm muscles. Biochem. J. 93, 652–665 (1964)
Reimer, F., Loffler, G., Hennig, G., Wieland, O. H.: The influence of insulin on glucose and fatty acid metabolism in the isolated perfused rat hind quarter. Hoppe-Seylers Z. physiol. Chem. 356, 1055–1066 (1975)
Rennie, M. J., Johnson, R. H.: Effects of an exercise-diet program on metabolic changes with exercise in runners. J. appl. Physiol. 37, 821–825 (1974)
Rennie, M. J., Winder, W. W., Holloszy, J. O.: A sparing effect of increased plasma fatty acids on muscle and liver glycogen in the exercising rat. Biochem. J. 156, 647–655 (1976)
Saltin, B., Hermansen, L.: Glycogen stores and prolonged severe exercise. In: Symposia of the Swedish Nutrition Foundation, Vol. V. (G. Blix, ed.), pp. 32–46. Uppsala: Almquist and Wiksell 1967
Shipp, J. C.: Interrelation between carbohydrate and fatty acid metabolism of isolated perfused rat heart. Metabolism 13, 852–866 (1964)
Weber, G., Convery, H. J. H., Lea, M. A., Stamm, N. B.: Feedback inhibition of key glycolytic enzymes in liver: action of free fatty acids. Science 154, 1357–1360 (1966)
Williams, C., Maughan, R. J., Kelman, G. R., Campbell, D. M., Hepburn, D.: Fat mobilisation during exercise following dietary and exercise-induced changes in muscle glycogen. Proc. Nutr. Soc. 35, 135A (1976)
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R. J. M. is in receipt of a Science Research Council Postgraduate Studentship award
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Maughan, R.J., Williams, C., Campbell, D.M. et al. Fat and carbohydrate metabolism during low intensity exercise: Effects of the availability of muscle glycogen. Europ. J. Appl. Physiol. 39, 7–16 (1978). https://doi.org/10.1007/BF00429674
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DOI: https://doi.org/10.1007/BF00429674