Archives of Microbiology

, Volume 114, Issue 1, pp 51–54 | Cite as

The effect of inorganic phosphate on cyanogenesis by Pseudomonas aeruginosa

  • R. Meganathan
  • P. A. Castric


The biosynthesis of hydrogen cyanide (HCN) by a strain of Pseudomonas aeruginosa is found to be significantly influenced by inorganic phosphate. Optimum HCN production occurs when the phosphate concentration is between 1 and 10 mM. Above and below this concentration the amount of HCN produced decreases sharply and at 0.1 and 100 mM phosphate low HCN production occurs. If a culture growing at 0.1 mM phosphate and producing low HCN is shifted to 10 mM phosphate, HCN biosynthesis resumes. Experiments with chloramphenicol indicate that de novo-protein synthesis is required for the process.

Key words

Hydrogen cyanide biosynthesis Pseudomonas aeruginosa Phosphate effect on HCN Secondary metabolism 


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  1. Aldridge, W. N.: A new method for the estimation of micro quantities of cyanide and thiocyanate. Analyst 69, 262–265 (1944)Google Scholar
  2. Brysk, M. N., Lauinger, C., Ressler, C.: Biosynthesis of cyanide from (2-14C15N) glycine in Chromobacterium violaceum. Biochim. biophys. Acta (Amst.) 184, 583–588 (1969)Google Scholar
  3. Castric, P. A.: Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa. Canad. J. Microbiol. 21, 613–618 (1975)Google Scholar
  4. Demain, A. L., Inamine, E.: Biochemistry and regulation of streptomycin and mannosidostreptomycinase (α-d-mannosidase) formation. Bact. Rev. 34, 1–19 (1970)Google Scholar
  5. Hou, C. I., Gronlund, F., Campbell, J. J. R.: Influence of phosphate starvation on cultures of Pseudomonas aeruginosa. J. Bact. 92, 851–855 (1966)Google Scholar
  6. Hutchinson, S. A.: Biological activities of fungal metabolites. Ann. Rev. Phytopath. 11, 223–246 (1973)Google Scholar
  7. Lorck, H.: Production of hydrocyanic acid by bacteria. Physiol. Plant. 1, 142–146 (1948)Google Scholar
  8. Mann, M. B., Huang, P. C.: New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low phosphate medium. J. Bact. 118, 209–212 (1974)Google Scholar
  9. Meganathan, R., Castric, P. A.: Effect of inorganic phosphate on hydrogen cyanide biosynthesis by Pseudomonas aeruginosa. Abstracts of the Ann. Soc. Microbiol., p. 141 (1976)Google Scholar
  10. Michaels, R., Corpe, W. A.: Cyanide formation by Chromobacterium violaceum. J. Bact. 89, 106–112 (1965)Google Scholar
  11. Miller, A. V., Walker, J. B.: Accumulation of streptomycin phosphate in cultures of streptomycin producers grown an a highphosphate medium. J. Bact. 104, 8–12 (1970)Google Scholar
  12. Patty, A. F.: The production of hydrocyanic acid by Bacillus pyocyaneus. J. infect. Dis. 29, 73–77 (1921)Google Scholar
  13. Robbers, J. E., Robertson, L. W., Hornemann, K. M., Jundra, A., Floss, H. G.: Physiological studies on ergot: further studies on the induction of alkaloid synthesis by Tryptophan and its inhibition by phosphate. J. Bact. 112, 791–796 (1972)Google Scholar
  14. Weinberg, E. D.: Secondary metabolism: Raison d'être. Persp. Biol. Med. 14, 565–577 (1971)Google Scholar
  15. Weinberg, E. D.: Secondary metabolism: Control by temperature and inorganic phosphate. Develop. Industr. Microbiol. 15, 70–81 (1974)Google Scholar
  16. Wissing, F.: Growth curves and pH optima for cyanide producing bacteria. Physiol. Plant. 21, 589–593 (1968)Google Scholar
  17. Wissing, F.: Cyanide production from glycine by a homogenate from a Pseudomonas species. J. Bact. 121, 695–699 (1975)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • R. Meganathan
    • 1
  • P. A. Castric
    • 1
  1. 1.Department of Biological SciencesDuquesne UniversityPittsburghUSA

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