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Studies on the cell cycle of Myxobacter AL-1

II. Activities of seven enzymes during the cell cycle

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Abstract

The properties of seven enzymes were studied in extracts from Myxobacter AL-1. The enzymes were isocitrate dehydrogenase (E.C. 1.1.1.42), succinate dehydrogenase (E.C. 1.3.99.1), alkaline phosphatase (E.C. 3.1.3.1), α-glucosidase (E.C. 3.2.1.20), β-glucosidase (E.C. 3.2.1.21), β-galactosidase (E.C. 3.2.1.23), and N-acetyl-glucosaminidase (E.C. 3.2.1.30). Four of these enzymes: isocitrate dehydrogenase, α-glucosidase, β-glucosidase, and β-galactosidase are cytosolic enzymes. Succinate dehydrogenase was found to be located on the cytoplasmic membrane system, whereas alkaline phosphatase and N-acetyl-glucosaminidase were considered as enzymes which bind the outer membranes resp. the cell wall. During the cell cycle, all enzymes have a pattern of discontinuous activity increase. Succinate dehydrogenase and isocitrate dehydrogenase exhibit a stepwise increase of activity, whereas the other enzymes follow the pattern of a peak enzyme.

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References

  • Birnie, G. D., Rickwood, D., Hell, A.: Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamide. Biochim. biophys. Acta (Amst.) 331, 283–294 (1973)

    Google Scholar 

  • Boll, M.: Enzyme der Elektronentransportpartikel aus Rhodospirillum rubrum: Eigenschaften von NADH-Succinat-Cytochrom-c-Reductase. Arch. Mikrobiol. 64, 85–102 (1968)

    Google Scholar 

  • Borooah, J., Leabeck, D. H., Walker, P. G.: Studies on glucosaminidase. II. Substrates for N-acetylglucosaminidase. Biochem. J. 78, 106–110 (1961)

    Google Scholar 

  • Brewer, G. J.: An introduction to isozyme techniques, pp. 97–98. New York: Academic Press 1970

    Google Scholar 

  • Duerksen, J. D., Halvorson, H. O.: Purification and properties of an inducible β-glucosidase of yeast. J. biol. Chem. 233, 1113–1120 (1958)

    Google Scholar 

  • Garen, A., Levinthal, C.: A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of Escherichia coli. I. Purification and characterization of alkaline phosphatase. Biochim. biophys. Acta (Amst.) 38, 470–485 (1960)

    Google Scholar 

  • Halvorson, H. O.: α-Glucosidase from yeast. In: Methods in enzymology, Vol. 8 (E. F. Neufeld, V. Ginsburg, eds.), pp. 559–562. New York-London: Academic Press 1966

    Google Scholar 

  • Hock, B.: Die zeitliche Dauer der Isocitrat-Lyase-Synthese in Kotyledonen von Wassermelonen-Keimlingen. Planta (Berl.) 93, 26–38 (1970)

    Google Scholar 

  • Hüttermann, A., Guntermann, U.: Metrizamide in D2O-a novel solute for the separation of labeled and unlabeled proteins by equilibrium density gradient centrifugation. Analyt. Biochem. 64, 360–366 (1975)

    Google Scholar 

  • Hüttermann, A., Porter, M. T., Rusch, H. P.: Activity of some enzymes in Physarum polycephalum. I. In the growing plasmodium. Arch. Mikrobiol. 74, 90–100 (1970)

    Google Scholar 

  • Kaback, H. R., Stadtman, E. R.: Proline uptake by an isolated cytoplasmic membrane preparation of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 55, 920–927 (1966)

    Google Scholar 

  • Kleinig, H.: Membranes from Myxococcus fulvus (Myxobacteriales) containing carotenoid glucosides. I. Isolation and composition. Biochim. biophys. Acta (Amst.) 274, 489–498 (1972)

    Google Scholar 

  • Kramhøft, B., Nissen, S. B., Zeuthen, E.: The cell cycle in heat-and selection-synchronized Schizosaccharomyces pombe. Carlsberg Res. Commun. 41, 15–25 (1976)

    Google Scholar 

  • Lederberg, J.: The beta-d-Galactosidase of Escherichia coli, strain K 12. J. Bact. 60, 381–392 (1950)

    Google Scholar 

  • Lowry, H. O., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  • Miura, T., Mizushima, S.: Separation by density gradient centrifugation of two tapes of membranes from spheroplast membrane of Escherichia coli K 12. Biochim. biophys. Acta (Amst.) 150, 159–161 (1968)

    Google Scholar 

  • Miura, T., Mizushima, S.: Separation and properties of outer and cytoplasmic membranes in Escherichia coli. Biochim. biophys. Acta (Amst.) 193, 268–276 (1969)

    Google Scholar 

  • Oltman, L. F., Stouthamer, A. H.: Purification of cytoplasmic membranes and outer membranes from Proteus mirabilis. Arch. Mikrobiol. 93, 311–325 (1973)

    Google Scholar 

  • Osborn, M. J., Gander, J. E., Parisi, E., Carson, J.: Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J. biol. Chem. 247, 3962–3972 (1972)

    Google Scholar 

  • Rickwood, D.: Isopyenic centrifugation of nucleic acids and their interaction with nuclear proteins in iodinated density-gradient media. In: Biological separations in iodinated density gradient media (D. Rickwood, ed.), pp. 27–40. London: Information Retrieval 1976

    Google Scholar 

  • Sottocasa, G. L., Kuylenstierna, B., Eraster, L., Bergstrand, A.: Separation and some enzymatic properties of the inner and outer membranes of rat liver mitochondria. In: Methods in enzymology, Vol. X (S. P. Colowick, N. O. Kaplan, eds.), pp. 448–463. New York: Academic Press 1967

    Google Scholar 

  • Spencer, M. E., Guest, J. R.: Proteins of the inner membrane of Escherichia coli: Identification of succinate dehydrogenase by polyacrylamide gel electrophoresis with sdh amber mutants. J. Bact. 117, 947–953 (1973)

    Google Scholar 

  • Tan, I., Hartman, W., Guntermann, U., Hüttermann, A., Kühlwein, H.: Studies on the cell cycle of Myxobacter AL-1. I. Size fractionation of exponentially growing cells by zonal centrifugation. Arch. Microbiol. 100, 389–396 (1974)

    Google Scholar 

  • Tsukagoshi, N., Fox, C. F.: Hybridization of membranes by sonic irradiation. Biochemistry 10, 3309–3313 (1971)

    Google Scholar 

  • Voelz, H., Ortigoza, R. O.: Cytochemistry in Myxococcus xanthus. J. Bact. 96, 1357–1365 (1968)

    Google Scholar 

  • White, D. A., Lennarz, W. J., Schnaitman, C. A.: Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli. J. Bact. 109, 686–690 (1972)

    Google Scholar 

  • Wrigley, C. W.: Gel-electrofocusing—a technique for analyzing multiple protein samples by isoelectric focusing. Sci. Tools 15, 17–23 (1968)

    Google Scholar 

  • Yasphe, J., Halvorson, H. O.: β-d-Galactosidase activity in single yeast cells during cell cycle of Saccharomyces lactis. Science 191, 1283–1284 (1976)

    Google Scholar 

  • Zusman, D., Gottlieb, P., Rosenberg, E.: Division cycle of Myxococcus xanthus. III. Kinetics of cell growth and protein synthesis. J. Bact. 105, 811–819 (1971)

    Google Scholar 

  • Zusman, D., Rosenberg, E.: DNA cycle of Myxococcus xanthus. J. molec. Biol. 49, 609–619 (1970)

    Google Scholar 

  • Zusman, D., Rosenberg, E.: Division cycle of Myxococcus xanthus. II. Kinetics of stable and unstable ribonucleic acid synthesis. J. Bact. 105, 801–810 (1971)

    Google Scholar 

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Hartmann, W., Tan, I., Hüttermann, A. et al. Studies on the cell cycle of Myxobacter AL-1 . Arch. Microbiol. 114, 13–18 (1977). https://doi.org/10.1007/BF00429624

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