Virchows Archiv A

, Volume 395, Issue 2, pp 153–168 | Cite as

Immunoelectron microscopic study of plasma protein pathways through the different segments of the rat intestinal vasculature

  • Nicole Hinglais
  • Jeannine Grossetete
  • Michel Paing
  • Jean Bariety
Original Articles


Nineteen Brown-Norway (BN) rats received intravenous injections of sheep anti-peroxidase (HRP) antibodies. Four BN rats were immunized to HRP. The anti-HRP antibodies were used to trace permeability pathways of large physiological molecules across different vessels of the small intestine. This organ was chosen because of the possibility of convenient “in situ” fixation and for the diversity of vessel types it contains.

It was shown that:
  1. 1)

    There were no obvious transendothelial pathways in arteries with an elastic lamina.

  2. 2)

    The antibodies readily crossed fenestrated capillaries through the fenestrae.

  3. 3)

    There were two possible pathways through muscle capillaries and pericytic venules, namely transcytoplasmic vesicular “cactus-like” channels and interendothelial junctions.

  4. 4)

    Interendothelial permeability was a possible factor in veins with an elastic lamina.

  5. 5)

    Lymphatics were readily permeable through intercellular junctions and cytoplasmic vesicles.


Key Words

Immunoelectron microscopy Plasma proteins Transvascular pathway 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Nicole Hinglais
    • 1
  • Jeannine Grossetete
    • 1
  • Michel Paing
    • 1
  • Jean Bariety
    • 1
  1. 1.Groupe de Recherches sur la Pathologie Rénale et VasculaireINSERM U 28, CNRS ERA 48, Hôpital BroussaisParis Cédex 14France

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