, Volume 155, Issue 4, pp 350–355 | Cite as

Growth regulators, Rhizobium and nodulation in peas

The cytokinin content of a wild-type and a Ti-plasmid-containing strain of R. leguminosarum
  • T. L. Wang
  • E. A. Wood
  • N. J. Brewin


The cytokinin content of roots and nodules of pea and the culture supernatants from two strains of Rhizobium leguminosarum has been examined. Roots, nodules and wild-type Rhizobium culture medium contained very little cytokinin as indicated by bioassay. Chemical ionisation gas chromatography-mass spectrometric analysis of the isopentenyladenine content of the culture medium from the Rhizobium strains confirmed that the content of the wild-type was low (approx. 1 ng dm-3) but that it was increased by the introduction of the Agrobacterium Ti plasmid into the Rhizobium strain.

Key words

Cytokinin Root nodules Pisum Rhizobium Ti prasmid 



chemical ionisation


gas chromatography-mass spectrometry


high performance liquid chromatography




multiple ion monitoring


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  1. Arora, N., Skoog, F., Allen, O.N. (1959) Kinetin-induced pseudonodules on tobacco roots. Am. J. Bot. 46, 610–613Google Scholar
  2. Beringer, J.E. (1974) R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84, 188–198Google Scholar
  3. Beynon, J.L., Beringer J.E., Johnston, A.W.B. (1980) Plasmids and host range in Rhizobium leguminosarum and Rhizobium phaseoli. J. Gen. Microbiol. 120, 421–429Google Scholar
  4. Biddington, N.L., Thomas, T.H. (1973) A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts. Planta 111, 183–186Google Scholar
  5. Elliot, D.C. (1979) Ionic regulation for cytokinin-dependent betacyanin synthesis in Amaranthus seedlings. Plant Physiol. 63, 264–268Google Scholar
  6. Greene, E.M. (1980) Cytokinin production by microorganisms. Bot. Rev. 46 25–74Google Scholar
  7. Henson, I.E., Wheeler, C.T. (1976) Hormones in plants bearing nitrogen-fixing root nodules: the distribution of cytokinins in Vicia faba L. New Phytol. 76, 433–439Google Scholar
  8. Hombrecher, G., Brewin, N.J., Johnston, A.W.B. (1981) Linkage of genes for nitrogenase and nodulation ability on plasmids of Rhizobium leguminosarum and R. phaseoli. Mol. Gen. Genet. 182, 133–136Google Scholar
  9. Hooykaas, P.J.J. (1979) The role of plasmid determinated functions in the interactions of Rhizobiaceae with plant cells: a genetic approach. Ph.D. thesis, University of Leiden, NetherlandsGoogle Scholar
  10. Hooykaas, P.J.J., Klapwijk, P.M., Nuti, M.P., Schilperoort, R.A., Rörsch, A. (1977) Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent agrobacteria and to Rhizobium ex planta. J. Gen. Microbiol. 98, 477–484Google Scholar
  11. Hooykaas, P.J.J., Van Brussel, A.N.N., Den Dulk-Ras, H., Van Sloyteren, G.M.S., Schilperoort, R.A. (1981) Sym plasmid of Rhizobium trifolii expressed in different rhizobial species and Agrobacterium tumefaciens Nature (London) 291, 351–354Google Scholar
  12. Summons, R.E., Letham, D.S., Gollnow, B.I., Parker, C.W., Entsch, B., Johnson, L.P., Macleod, J.K., Rolfe, B.G. (1981) Cytokinin translocation and metabolism in species of the Leguminoseae: Studies in relation to shoot and nodule development. In: Metabolism and molecular activities of cytokinins, pp. 69–79, Guern, J., Peaud-Lenoel, C., eds. Springer, Berlin Heidelberg New YorkGoogle Scholar
  13. Syono K., Torrey, J.G. (1976) Identification of cytokinins of root nodules of the garden pea, Psium sativum L. Plant Physiol. 57, 602–606Google Scholar
  14. Torrey, J.G. (1961) Kinetin as a trigger for mitosis. Exp. Cell Res. 23, 281–299Google Scholar
  15. Wang, T.L., Beutelmann, P., Cove, D.J. (1981a) Cytokinin biosynthesis in mutants of the moss, Physcomitrelia patens. Plant Physiol. 68, 739–744Google Scholar
  16. Wang, T.L., Horgan, R., Cove, D.J. (1981b) Cytokinins from the moss, Physcomitrella pattens. Plant Physiol. 68, 735–738Google Scholar
  17. Wang, T.L., Wood, E.A., Brewin, N.J. (1982) Growth regulators, Rhizobium and nodulation in peast. Indole-3-acetic acid from the culture medium of nodulating and non-nodulating strains of R. leguminosarum. Planta 155, 345–349Google Scholar
  18. Young, H. (1977) Identification of cytokinins from natural sources by gas-liquid chromatography/mass spectrometry. Anal. Biochem. 79, 226–233Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • T. L. Wang
    • 1
  • E. A. Wood
    • 1
  • N. J. Brewin
    • 1
  1. 1.John Innes InstituteNorwichUK

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