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Planta

, Volume 155, Issue 4, pp 350–355 | Cite as

Growth regulators, Rhizobium and nodulation in peas

The cytokinin content of a wild-type and a Ti-plasmid-containing strain of R. leguminosarum
  • T. L. Wang
  • E. A. Wood
  • N. J. Brewin
Article

Abstract

The cytokinin content of roots and nodules of pea and the culture supernatants from two strains of Rhizobium leguminosarum has been examined. Roots, nodules and wild-type Rhizobium culture medium contained very little cytokinin as indicated by bioassay. Chemical ionisation gas chromatography-mass spectrometric analysis of the isopentenyladenine content of the culture medium from the Rhizobium strains confirmed that the content of the wild-type was low (approx. 1 ng dm-3) but that it was increased by the introduction of the Agrobacterium Ti plasmid into the Rhizobium strain.

Key words

Cytokinin Root nodules Pisum Rhizobium Ti prasmid 

Abbreviations

CI

chemical ionisation

GCMS

gas chromatography-mass spectrometry

HPLC

high performance liquid chromatography

iPAde

isopentenyladenine

MIM

multiple ion monitoring

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • T. L. Wang
    • 1
  • E. A. Wood
    • 1
  • N. J. Brewin
    • 1
  1. 1.John Innes InstituteNorwichUK

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