Abstract
Two new methane-producing cocci have been isolated from separate sediment enrichments using a defined synthetic growth medium. A freshwater methanogen, Methanogenium olentangyi, sp. nov., was isolated from Olentangy River sediment, Columbus, Ohio. The organism was a non-motile, irregular coccus that produced methane from CO2 plus H2, and required acetate for growth. A marine methanogen, Methanococcus deltae, sp. nov., was isolated from Gulf of Mexico sediments and was a non-motile, irregular coccus that grew on CO2 plus H2, or on formate. The guanine-plus-cytosine content of the deoxyribonucleic acid of the isolates was 54.4 and 40.5 mol%, respectively.
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References
Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
Balch WE, Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32:781–791
Conway de Macario E, Macario AJL, Wolin MJ (1982) Specific antisera and immunological procedures for characterization of methanogenic bacteria. J Bacteriol 149:320–328
Conway de Macario E, Wolin MJ, Macario AJL (1982a) Antibody analysis of relationships among methanogenic bacteria. J Bacteriol 149:316–319
Conway de Macario E, Wolin MJ, Macario AJL (1981) Immunology of Archaebacteria that produce methane gas. Science 214:74–75
Corder RE, Hook LA, Frea JI (1982) Isolation and characterization of two new methane producing cocci. Abstr Amer Soc Microbiol, Washington, D. C., p 111
DeLey J, Frateur J (1974) Genus Acetobacter Beijerinck 1898. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of determinative bacteriology 8th ed. Williams and Wilkins Co, Baltimore, MD, pp 276–278
Ferguson TJ, Mah RA (1982) Isolation and characterization of a formate and H2/CO2 utilizing thermophilic methanogen. Abstr Amer Soc Microbiol, Washington, D.C., p 110
Fox GE, Magrum LJ, Balch WE, Wolfe RS, Woese CR (1977) Classification of methanogenic bacteria by 16S ribosomal RNA characterization. Proc Natl Acad Sci USA 74:4537–4541
Gibson T, Gordon RE (1974) Gerus I. Bacillus Cohn 1872. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of determinative bacteriology, 8th ed. Williams and Wilkins Co., Baltimore, MD, pp 529–550
Holdeman LV, Moore WEC (1974) Genus I. Bacteriodes Castillani and Chalmers 1919. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of determinative bacteriology, 8th ed. Williams and Wilkins Co., Baltimore, MD, pp 385–404
Jones WJ, Paynter MJB (1981) Characterization of Methanogenium paludismaritimae sp. nov., a marine methanogen. Abstr Amer Soc Microbiol, Washington, D.C., p 111
Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218
Rivard CJ, Smith PH (1982) Isolation and characterization of a thermophilic marine methanogen Methanogenium thermophilicum sp. n.. Abstr Amer Soc Microbiol, Washington, D.C., p 111
Romesser JA, Wolfe RS, Mayer F, Spiess E, Walther-Mauruschat A (1979) Methanogenium, a new genus of marine methanogenic bacteria, and characterization of Methanogenium cariaci sp. nov. and Methanogenium marisnigri sp. nov. Arch Microbiol 121:147–153
Schildkrant CL, Marmur J, Doty P (1962) Determination of base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol 4:430–443
Stadtman TC, Barker HA (1951) Studies on methane fermentation.X. A new formate-decomposing bacterium, Methanococcus vannielli. J Bacteriol 62:269–280
Whitman WB, Ankwanda E, Wolfe RS (1982) Nutrition and carbon metabolism of Methanococcus voltae. J Bacteriol 149:852–863
Zeikus JG (1977) The biology of methanogenic bacteria. Bacteriol Rev 41:514–541
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Corder, R.E., Hook, L.A., Larkin, J.M. et al. Isolation and characterization of two new methane-producing cocci: Methanogenium olentangi, sp. nov., and Methanococcus deltae, sp. nov.. Arch. Microbiol. 134, 28–32 (1983). https://doi.org/10.1007/BF00429402
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DOI: https://doi.org/10.1007/BF00429402