, Volume 27, Issue 4, pp 349–357 | Cite as

Effect of subchronic treatment with (−) δ8-trans-tetrahydrocannabinol (δ8-THC) on food intake, body temperature, hexobarbital sleeping time and hexobarbital elimination in rats

  • Dietz Rating
  • Ina Broermann
  • Henning Honecker
  • Sieglinde Kluwe
  • Helmut Coper
Animal Studies


In a series of experiments in rats it was possible to prove that δ8-THC has the same type of effectiveness as the stereo-isomeric δ9-THC.

Body temperature was reduced to 35.5‡ C 90 min after injection of 10 mg/kg δ8-THC; 5 mg/kg δ8-THC extended the duration of hexobarbital sleeping time from 119 min in control animals to 205 min. Following treatment with δ8-THC for 9 and 10 days the effects on hypothermia and prolongation of sleeping time were less pronounced than in acute experiments. Body temperature dropped to 36.4‡ C 90 min p.i. and the duration of hexobarbital sleeping time was only extended to 156 min. This attenuation of effects after subchronic THC treatment was regarded as due to the development of tolerance.

In order to exclude the possibility that the extension of sleeping time might be due to an effect of THC on hexobarbital metabolism, the hexobarbital concentration in cerebral tissue and blood was determined 30, 60, 90 and 120 min after the administration of 100 mg/kg hexobarbital-Na. There was no difference in hexobarbital elimination between rats treated with THC in a single dose or over a 10-day period and control animals.

Key words

δ8-THC Hexobarbital Tolerance 


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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Dietz Rating
    • 1
  • Ina Broermann
    • 1
  • Henning Honecker
    • 1
  • Sieglinde Kluwe
    • 1
  • Helmut Coper
    • 1
  1. 1.Institut für NeuropsychopharmakologieFreie UniversitÄt BerlinFederal Republik of Germany

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