Archives of Microbiology

, Volume 112, Issue 2, pp 163–168 | Cite as

Acidostability of spheroplasts prepared from Thiobacillus thiooxidans

  • Akinorl Noguchi
  • Michihiro Takama
  • Takeshi Sekiguchi
  • Noriyuki Koyama
  • Yoshiaki Nosoh
Article

Abstract

Thiobacillus thiooxidans was acidostable even in the absence of its respiratory substrate, elementary sulfur. This suggests that the acidostability of the bacterium was energy-independent. The organism was subjected to osmotic shock with 0.75 M sucrose at 0°C and then treated with snail intestinal juice in the presence of 0.3 M sucrose. The decrease in the optical density of the sample thus prepared on dilution with deionized water and electron microscopic observation of the sample showed that spheroplasts were formed from the bacterium by this procedure. Spheroplasts were able to respire sulfur and their respiratory activity was acidostable. Spheroplasts, when treated with Nagase, proteolytic enzyme, lost their acidostability, and some protein components disappeared from the membrane fraction. This suggests that the acidostability of the bacterium may be related to protein components of the membrane.

Key words

Thiobacillus thiooxidans Sulfur oxidizing bacterium Loss of acidostability Spheroplast formation Snail intestinal juice Osmotic shock Proteinase treatment Membrane proteins 

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Akinorl Noguchi
    • 1
  • Michihiro Takama
    • 1
  • Takeshi Sekiguchi
    • 1
  • Noriyuki Koyama
    • 1
  • Yoshiaki Nosoh
    • 1
  1. 1.Laboratory of Chemistry of Natural ProductsTokyo Institute of TechnologyTokyoJapan

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